FlyBase Allele Report: Dmel\IKKε[NIG.2615R]

General Information

Symbol

Dmel\IKKε^NIG.2615R

Species

D. melanogaster

Name

FlyBase ID

FBal0414313

Feature type

allele

Associated gene

Dmel\IKKε

Associated Insertion(s)

Carried in Construct

P{NIG.2615R}

Also Known As

ikkε^RNAi, IR2615R

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21, Kuranaga et al., 2006, Oshima, 2006)

Progenitor genotype

Carried in construct

P{NIG.2615R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-2615R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

(Lee et al., 2009)

visible | adult stage, with Scer\GAL4^sca.PU

(Otani et al., 2015)

Phenotype Manifest In

actin filament bundle, with Scer\GAL4^ap-md544

(Otani et al., 2011)

chaeta, with Scer\GAL4^ap-md544

(Otani et al., 2011)

dendrite & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4^ppk.PG

(Lee et al., 2009)

macrochaeta, with Scer\GAL4^ap-md544

(Oshima et al., 2006)

macrochaeta, with Scer\GAL4^sca-P309

(Koto et al., 2009)

mesothoracic bristle, with Scer\GAL4^sca.PU

(Otani et al., 2015)

microtubule & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4^ppk.PG

(Lee et al., 2009)

terminal tracheal cell, with Scer\GAL4^btl.PS

(Oshima et al., 2006)

Detailed Description

Statement

Reference

Expression of IKKε^NIG.2615R under the control of Scer\GAL4^sca.PU results in abnormal bristle morphology, wherein bristles are short, branched and have a characteristic swollen region.

(Otani et al., 2015)

Bristles are short and branched in flies expressing ik2^NIG.2615R under the control of Scer\GAL4^ap-md544. Ultrastructurally, the actin bundles of the bristles are disorganised and some run through the cytoplasm. Actin bundle area and size are both significantly reduced compared to wild type, whereas the number of actin bundles is significantly increased. The number of microtubules is not significantly altered, and most retain the orientation parallel to the bristles' long axis. There is a significant increase in vesicle density and diameter within the bristles. Multivesicular bodies are rarely seen and there is a modest increase in mitochondria compared to wild type.

(Otani et al., 2011)

Bristles are shorter and thicker in the adult notum of Scer\GAL4^sca-P309; ik2^{dsRNA.Scer\UAS} flies.

(Koto et al., 2009)

The proximal dendrites remain intact for most ddaC neurons at 18 hours after puparium formation (APF) in animals expressing ik2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ppk.PG, indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals).

The microtubules remain intact in the dendrites of ddaC neurons at 6 hours after puparium formation (APF) in animals expressing ik2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ppk.PG (microtubules in the dendrites of wild-type ddaC neurons show breakage at this stage).

(Lee et al., 2009)

Expression of ik2^{dsRNA.Scer\UAS} in S2 cells, under the control of Scer\GAL4^Act5C.PI, does not affect cell viability. However, these S2 cells show an increased likelihood to have a serrate or stellate morphology, instead of the typical round morphology.

Embryos treated with ik2 RNAi, generated by expression of ik2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^btl.PS, show tracheal terminal branching defects. These include the duplication of terminal cells and the misorientation of the actin core.

Flies treated with ik2 RNAi, generated by expression of ik2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ap-md544, show gnarled, forked macrochaetae.

(Oshima et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

IKKε^NIG.2615R, Scer\GAL4^sca.PU has visible | adult stage phenotype, non-enhanceable by spn-F¹/spn-F¹

(Otani et al., 2015)

NOT suppressed by

Statement

Reference

IKKε^NIG.2615R, Scer\GAL4^sca.PU has visible | adult stage phenotype, non-suppressible by spn-F¹/spn-F¹

(Otani et al., 2015)

NOT Enhancer of

Statement

Reference

IKKε^NIG.2615R/Scer\GAL4^sca.PU is a non-enhancer of visible | adult stage phenotype of spn-F¹

(Otani et al., 2015)

NOT Suppressor of

Statement

Reference

IKKε^NIG.2615R/Scer\GAL4^sca.PU is a non-suppressor of visible | adult stage phenotype of spn-F¹

(Otani et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

IKKε^NIG.2615R, Scer\GAL4^sca-P309 has macrochaeta phenotype, enhanceable by Diap1^UAS.Tag:FLAG, Scer\GAL4^sca-P309

(Koto et al., 2009)

IKKε^NIG.2615R, Scer\GAL4^sca-P309 has macrochaeta phenotype, enhanceable by Dronc^{C318G.UAS.EGFP}, Scer\GAL4^sca-P309

(Koto et al., 2009)

NOT Enhanced by

Statement

Reference

IKKε^NIG.2615R, Scer\GAL4^sca.PU has mesothoracic bristle phenotype, non-enhanceable by spn-F¹/spn-F¹

(Otani et al., 2015)

NOT suppressed by

Statement

Reference

IKKε^NIG.2615R, Scer\GAL4^sca.PU has mesothoracic bristle phenotype, non-suppressible by spn-F¹/spn-F¹

(Otani et al., 2015)

NOT Enhancer of

Statement

Reference

IKKε^NIG.2615R/Scer\GAL4^sca.PU is a non-enhancer of mesothoracic bristle phenotype of spn-F¹

(Otani et al., 2015)

NOT Suppressor of

Statement

Reference

IKKε^NIG.2615R/Scer\GAL4^sca.PU is a non-suppressor of mesothoracic bristle phenotype of spn-F¹

(Otani et al., 2015)

Other

Statement

Reference

BacA\p35^UAS.cHa, IKKε^NIG.2615R, Scer\GAL4^sca-P309 has macrochaeta phenotype

(Koto et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

spn-F¹/spn-F¹ does not suppress or enhance the abnormal bristle morphology seen in flies expressing IKKε^NIG.2615R under the control of Scer\GAL4^sca.PU, and vice versa.

(Otani et al., 2015)

Overexpression of th^{Scer\UAS.T:Zzzz\FLAG} enhances the short-bristle phenotype of Scer\GAL4^sca-P309; ik2^{dsRNA.Scer\UAS} flies.

Co-expression of Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP} in Scer\GAL4^sca-P309; ik2^{dsRNA.Scer\UAS} flies significantly enhances the short-bristle phenotype.

(Koto et al., 2009)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa and ik2^{dsRNA.Scer\UAS}, both under the control of Scer\GAL4^sca-P309 generates a short bristle phenotype, as found in Scer\GAL4^sca-P309; ik2^{dsRNA.Scer\UAS} mutants.

(Koto et al., 2009)

Complementation and Rescue Data

Rescues

IKKε^NIG.2615R/Scer\GAL4^sca.PU rescues IKKε^UAS.Tag:MYC

(Otani et al., 2015)

Comments

Co-expression of IKKε^NIG.2615R rescues the bristle loss seen in flies expressing IKKε^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^sca.PU.

(Otani et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

2615R-1

P{NIG.2615R}1

2615R-2

P{NIG.2615R}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

IKKε^NIG.2615R

IKKε^RNAi.UAS

IKKε^RNAi

(Otani et al., 2016)

IKKε^{dsRNA.Scer\UAS}

IKKε^dsRNA.UAS

ik2^NIG.2615R

ik2^{dsRNA.Scer\UAS}

ikkε^RNAi

(Otani et al., 2015)

Name Synonyms

Secondary FlyBase IDs

FBal0194832
FBal0273152

References (12)

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