A TI{KozakGAL4} DNA cassette has been inserted into Spn31A, replacing most of the coding sequence (coordinates of deleted sequence are 2L:10060860..10063791 , release 6 genome, and the 3' end of the deletion is 46bp upstream of the stop codon). This results in a simultaneous knock-out of Spn31A plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of Spn31A (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: GTCGACTAAACGCTTGCGCTCGG and GGTCACATTCTCACTGCGAATGG.