FlyBase Allele Report: Mmus\Wld[S-dead.UAS]

General Information

Symbol

Mmus\Wld^S-dead.UAS

Species

M. musculus

Name

FlyBase ID

FBal0414753

Feature type

allele

Associated gene

Mmus\Wld

Associated Insertion(s)

Carried in Construct

P{UAS-wld.S-dead}

Also Known As

UAS-Wld^S-dead

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Schoenmann et al., 2010, Avery et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-wld.S-dead}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form of the fusion protein associated with the mouse 'Wld[S]' mutation. The 'Wld[S]' fusion protein is composed of the N-terminal 70 amino acids of Mmus\Ube4b, a unique 18 amino acid domain generated during the fusion event and the full-length sequence of Mmus\Nmnat1. In this transgene, an H24A amino acid replacement has been introduced into the fusion protein sequence (this mutation largely abolishes Mmus\Nmnat1 enzymatic activity).

(Avery et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Wld

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Or22a.8197

(Avery et al., 2009)

abnormal neuroanatomy | conditional, with Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2014)

Phenotype Manifest In

olfactory receptor neuron, with Scer\GAL4^Or22a.8197

(Avery et al., 2009)

wing vein L1 | conditional, with Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2014)

Detailed Description

Statement

Reference

Expression of one copy of Mmus\wld^{S-dead.Scer\UAS} under the control of Scer\GAL4^VGlut-OK371 has a moderate protective effect on L1 vein neurons following axotomy. Fragmentation of axons occurs more slowly than in wild type.

(Neukomm et al., 2014)

Expression of Mmus\wld^{H24A.Scer\UAS} in the ddaC neurons (using the Scer\GAL4^ppk.PG line) has no protective effect on dendritic pruning as when Mmus\wld^S.Scer\UAS is expressed.

(Schoenmann et al., 2010)

Expression of Mmus\wld^{S-dead.Scer\UAS} under the control of Scer\GAL4^Or22a.8197 has very little protective effect on olfactory receptor neurons following axotomy: 92% of olfactory receptor neuron axons have undergone Wallerian degeneration within 15 days of injury.

(Avery et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^ppk.PH/Mmus\Wld^S-dead.UAS is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP

(Ji et al., 2022)

Scer\GAL4^ppk.PH/Mmus\Wld^S-dead.UAS is a non-enhancer of decreased size | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP