The R26E07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
The R19B03 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
In a split-GAL4 system, ScerGAL4DBD.R26E07 drives expression in the gamma dorsal Kenyon cells, gamma main Kenyon cells, alpha'/beta' anterior-posterior Kenyon cells, alpha'/beta' middle Kenyon cells, alpha/beta posterior Kenyon cells, alpha/beta surface Kenyon cells and alpha/beta core Kenyon cells when combined with HsapRELAAD.R19B03, and in the alpha'/beta' anterior-posterior Kenyon cells and alpha'/beta' middle Kenyon cells when combined with HsapRELAAD.R35B12.
In a split-GAL4 system, HsapRELAAD.R19B03 drives expression in gamma dorsal Kenyon cells when combined with ScerGAL4DBD.R39A11 and in the gamma dorsal Kenyon cells, gamma main Kenyon cells, alpha'/beta' anterior-posterior Kenyon cells, alpha'/beta' middle Kenyon cells, alpha/beta posterior Kenyon cells, alpha/beta surface Kenyon cells and alpha/beta core Kenyon cells when combined with ScerGAL4DBD.R26E07.