The VT043656 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
The VT040012 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
ScerGAL4DBD.VT043656 labels approximately 100 cells per hemisphere - mostly LC10a with a small number of LC10b neurons - when in combination with HsapRELAAD.R35D04 and HsapRELAAD.R25H09, and approximately 100 LC10a neurons per hemisphere when in combination with HsapRELAAD.VT040012, approximately 60% of which are fruitless-expressing lobula columnar neuron LC10a that EcollexAfru.P1.
HsapRELAAD.VT040012 labels approximately 100 LC10a neurons per hemisphere when in combination with ScerGAL4DBD.VT043656, approximately 60% of which are fruitless-expressing lobula columnar neuron LC10a that EcollexAfru.P1.
Labels approximately 100 LC10a neurons per hemisphere.