The GMR52F12 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
The GMR60E02 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
In a split-GAL4 system, HsapRELAAD.R52F12 drives expression almost exclusively in two distinct clusters of 40-50 zone C/E Johnston organ neurons when combined with ScerGAL4DBD.R60E02.
In a split-GAL4 system, ScerGAL4DBD.R60E02 drives expression almost exclusively in two distinct clusters of 40-50 zone C/E Johnston organ neurons when combined with HsapRELAAD.R52F12, and in these two clusters and multiple neurons elsewhere in the adult brain and thoracico-abdominal ganglion when combined with HsapRELAAD.R39A11.
Labels two distinct clusters of 40-50 zone C/E Johnston organ neurons.