The VT044845 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
The VT050660 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
In a split-GAL4 system, ScerGAL4DBD.VT044845 drives expression in adult moonwalker descending neuron, and one unidentified cell in the peripheral nervous system when combined with HsapRELAAD.VT050660, and in adult moonwalker descending neuron, one unidentified cell in the adult brain and two unidentified cells in the thoracico-abdominal ganglion when combined with HsapRELAAD.VT037220.
In a split-GAL4 system, HsapRELAAD.VT050660 drives expression in adult moonwalker ascending neuron, adult moonwalker descending neuron and two unidentified cells in the adult brain when combined with ScerGAL4DBD.VT049484, all these previous cells and two unidentified cells in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.VT037220, adult moonwalker descending neuron and one unidentified cell in the peripheral nervous system when combined with ScerGAL4DBD.VT044845, adult moonwalker descending neuron when combined with ScerGAL4DBD.VT014014, in adult moonwalker descending neuron and two unidentified cells in the adult brain when combined with ScerGAL4DBD.VT008453, and in adult moonwalker descending neuron, one unidentified cell in the adult brain and three unidentified cells in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.VT019059.