FlyBase Split System Combination Report: Scer\GAL4[DBD.R94F11]∩Hsap\RELA[AD.R24E12]

General Information

Symbol

Scer\GAL4^DBD.R94F11∩Hsap\RELA^AD.R24E12

FlyBase ID

FBco0001798

Feature Type

split system combination

Component Alleles

Hsap\RELA^AD.R24E12

Scer\GAL4^DBD.R94F11

Combination components

Component allele Hsap\RELA^AD.R24E12

Component

Use(s)

Regulatory region(s)

GMR24E12

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The R24E12 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Component allele Scer\GAL4^DBD.R94F11

Component

Use(s)

Regulatory region(s)

GMR94F11

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Description

The GMR94F11 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Expression Data

Reporters Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

dopaminergic PAM neuron 13

(Shuai et al., 2015)

dopaminergic PAM neuron 14

(Shuai et al., 2015)