The GMR30B01 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
The GMR31H10 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
In a split-GAL4 system, HsapRELAAD.R30B01 drives expression in 5-10 (out of 77) sensillum campaniformium neurons in the proximal part of the wing, a majority of distal 5-6 (out of 8) campaniformium neurons in the distal part of the wing, 7-10 (out of approximately 139) sensillum campaniformium neurons in the halteres and 3-5 (out of approximately 221) mechanosensory neurons that are part of the wing margin bristle when combined with ScerGAL4DBD.R31H10. Neurons on the wings and halteres send projections to the thoracico-abdominal ganglion, where some ascend to the adult subesophageal zone.
Labels 5-10 (out of 77) sensillum campaniformium neurons in the proximal part of the wing, a majority of distal 5-6 (out of 8) campaniformium neurons in the distal part of the wing, 7-10 (out of approximately 139) sensillum campaniformium neurons in the halteres and 3-5 (out of approximately 221) mechanosensory neurons that are part of the wing margin bristle.