FlyBase Split System Combination Report: Scer\GAL4[unc-4-DBD]∩Hsap\RELA[AD.R77C10]

General Information

Symbol

Scer\GAL4^unc-4-DBD∩Hsap\RELA^AD.R77C10

FlyBase ID

FBco0002108

Feature Type

split system combination

Component Alleles

Hsap\RELA^AD.R77C10

Scer\GAL4^unc-4-DBD

Combination components

Component allele Hsap\RELA^AD.R77C10

Component

Use(s)

Regulatory region(s)

GMR77C10

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The GMR77C10 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Component allele Scer\GAL4^unc-4-DBD

Component

Use(s)

Inserted element

TI{2A-GAL4(DBD)::Zip-}

promoter trap

Regulatory region(s)

unc-4

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Description

2A-GAL4(DBD)::Zip- is introduced into the attP site in the TI{TI}unc-4^FRT progenitor. The Trojan insertion introduces a stop codon, truncating the unc-4 protein after its first exon.

(Lacin et al., 2020)

Expression Data

Reporters Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

Notch OFF hemilineage neuron of NB7-4 lineage neuron

(Lacin et al., 2020)

Additional Information

Statement