FlyBase Split System Combination Report: Scer\GAL4[DBD.VT008681]∩Hsap\RELA[VGlut1-MI04979-Tp65AD.2]

General Information

Symbol

Scer\GAL4^DBD.VT008681∩Hsap\RELA^{VGlut1-MI04979-Tp65AD.2}

FlyBase ID

FBco0002179

Feature Type

split system combination

Component Alleles

Scer\GAL4^DBD.VT008681

Hsap\RELA^{VGlut1-MI04979-Tp65AD.2}

Combination components

Component allele Scer\GAL4^DBD.VT008681

Component

Use(s)

Regulatory region(s)

VT008681

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Description

The VT008681 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Component allele Hsap\RELA^{VGlut1-MI04979-Tp65AD.2}

Component

Use(s)

Inserted element

Mi{Trojan-p65AD.2}

gene trap

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The attP cassette in the Mi{MIC}VGlut^MI04979 insertion has been replaced with a Trojan p65AD cassette that contains a splice acceptor site followed by the T2A peptide and the p65(AD)::Zip+ component of the SplitGAL4 system, and an Hsp70 transcription termination signal.

(Lacin et al., 2019)

Expression Data

Reporters Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult lateral accessory lobe-wedge-ipsilateral nodulus 2 neuron

(Lu et al., 2022)

Additional Information

Statement