FlyBase Gene Report: Dmel\ImpL1

General Information

Symbol

Dmel\ImpL1

Species

D. melanogaster

Name

Ecdysone-inducible gene L1

Annotation Symbol

CG10717

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001256

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Red Book
Interactive Fly

Also Known As

Imp-L1

Key Links

Genomic Location

Cytogenetic map

70A7-70A7

Sequence location

3L:13,382,765..13,383,999 [+]

Recombination map

3-40

RefSeq locus

NT_037436 REGION:13382765..13383999

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (1 term)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in extracellular space

inferred from sequence model

(Natzle et al., 1992)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Kazal domain superfamily

Summaries

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Imp-L: Imp-Late

Imp-L encoding transcripts associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. Imp-L denotes three "late" genes involved in the eversion to the exterior of the elongated regions of discs by means of local changes in cell shape (Osterbur et al., 1988).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ImpL1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KUJ4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ImpL1-RA

FBtr0075842

NM_079334

1160

341

ImpL1-RB

FBtr0075843

NM_168541

1230

366

ImpL1-RC

FBtr0301405

NM_001169968

1157

340

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ImpL1-PA

FBpp0075578

38.3

341

8.13

FBpp0075579

41.0

366

8.58

FBpp0290619

38.2

340

8.13

Q6NL75

NP_001163439

ACZ94710

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Kazal domain superfamily

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ImpL1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.32

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage P1 -- prepupal stage P4(i)

organism

(Ritter and Beckstead, 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ImpL1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ImpL1

View exonic expression by tissue for Dmel\ImpL1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001256

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39499/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001256

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001256

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001256

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10717

Flygut - An atlas of the Drosophila adult midgut

FBgn0001256

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ImpL1

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ImpL1

Transgenic constructs containing regulatory region of ImpL1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ImpL1^GD3589

viable, with Scer\GAL4^Mef2.PR

ImpL1^GD3589

viable, with Scer\GAL4^pnr-MD237

ImpL1^GD3589

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ImpL1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

CG15721

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0001256

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-40

Cytogenetic map

70A7-70A7

Sequence location

3L:13,382,765..13,383,999 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

70A7-70A7

Limits computationally determined from genome sequence between P{PZ}l(3)04220⁰⁴²²⁰&P{lacW}l(3)j10B6^j10B6 and P{PZ}stv⁰⁰⁵⁴³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

70A-70A

(determined by in situ hybridisation)

(Natzle et al., 1992, Natzle et al., 1986)

Experimentally Determined Recombination Data

Location

3-40

(FlyBase, 2016)

3-34.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

55387

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04075}attP40

10717R-1

10717R-3

v30288

w¹¹¹⁸; P{GD3589}v30288/TM3

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (58)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_LP07125

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001256

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001256

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

Temporal profile of gene expression is altered in Eip74EF mutant background.

(Fletcher and Thummel, 1995)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

The sequential temporal expression of steroid hormone-responsive genes in imaginal discs may be important in organizing cellular mechanisms involved in morphogenesis of the epithelium.

(Natzle, 1993)

Sequence analysis of ImpL1 reveals structural features in the predicted gene product characteristic of secreted and membrane proteins.

(Natzle et al., 1992)

Encodes a transcript associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. A 'late' gene involved in the eversion to the exterior of the elongated regions of discs by means of local changes in cell shape (Osterbur, Fristrom, Natzle, Tojo and Fristrom, 1988).

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: ImpL1 CG10717

(Bayraktaroglu, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG10717

(Ni et al., 2010.12.1, Lebo et al., 2009, Haussmann et al., 2008)

IMP-L1

(Enya et al., 2014, McBrayer et al., 2007, Niwa et al., 2004, Chavez et al., 2000, Natzle et al., 2000, Natzle, 1993.1.6, Natzle et al., 1992, Vesenka and Natzle, 1990)

Imp-L1

(Ritter and Beckstead, 2010, Kozlova et al., 2009, King-Jones et al., 2005, Kedia et al., 2000, Bayer et al., 1996, Fletcher and Thummel, 1995, Andres et al., 1993, Natzle, 1993)

ImpL1

(Li et al., 2024, Huang et al., 2023, Yoo et al., 2021, Mateos et al., 2019, Meltzer et al., 2019, Xie et al., 2015, Deng and Kerppola, 2013, Japanese National Institute of Genetics, 2012.5.21, Zeitouni et al., 2007)

Name Synonyms

Ecdysone-inducible gene L1

(Lebo et al., 2009)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39499

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KUJ4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KUJ4

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001256

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39499/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001256

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001256

FlyMine - An integrated database for Drosophila genomics

FBgn0001256

InterPro - A database of protein families, domains and functional sites

Kazal domain superfamily

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10717

MARRVEL_MODEL - MARRVEL (model organism gene)

39499

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001256

DroID - A comprehensive database of gene and protein interactions.

FBgn0001256

DRSC - Results frm RNAi screens

FBgn0001256

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001256

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001256

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10717

Flygut - An atlas of the Drosophila adult midgut

FBgn0001256

References (54)

The following external sites may use different assemblies or annotations than FlyBase.

-0.32

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