ng-1, ngp, ng, SC1
Supported by strand-specific RNA-Seq data.
Gene model reviewed during 5.46
There is only one protein coding transcript and one polypeptide associated with this gene
107 (aa); 11.3 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ng1 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\ng1 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The binding properties of the ng-EcRE, a directly repeated half site (DR) located within the coding region of ng1, are studied to investigate the properties of EcREs composed of DRs. The ng-EcRE contacts the EcR/usp heterodimer through the DRs spaced by 12bp, this element may interact efficiently with Hr38 and Hr39. The ng-EcRE can promote functional interactions in vitro as well as in vivo, acting as a transcriptional enhancer able to confer a specific developmental expression profile to a minimal promoter in transgenic flies.
An ecdysone-responsive element is required for high level expression of the ng1 intermoult gene.
DNA-blotting assay has identified a high affinity ecdysone receptor binding site within the ng1 coding sequence. EMSA assay demonstrates the 93bp 'ng element' is able to bind an EcR/usp heterodimer and usp alone.