FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\ReepB
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General Information
Symbol
Dmel\ReepB
Species
D. melanogaster
Name
Receptor expression enhancing protein B
Annotation Symbol
CG8331
Feature Type
protein_coding_gene
FlyBase ID
FBgn0033906
Gene Model Status
Current
Stock Availability
15 publicly available
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
50E1-50E1
Sequence location
Recombination map
2-69
RefSeq locus
NT_033778 REGION:14170462..14172998
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in endoplasmic reticulum organization
inferred from mutant phenotype
(Yalçın et al., 2017)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in axon
inferred from direct assay
(Yalçın et al., 2017)
located_in endomembrane system
inferred from high throughput direct assay
(Tan et al., 2009)
located_in endoplasmic reticulum
inferred from direct assay
(Yalçın et al., 2017)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\ReepB for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A1Z9M2)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
ReepB-RA
FBtr0087525
NM_137092
771
178
ReepB-RB
FBtr0302451
NM_001201997
877
160
ReepB-RC
FBtr0302452
NM_001201998
1023
153
ReepB-RD
FBtr0302453
NM_001201999
846
178
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
ReepB-PA
FBpp0086654
20.3
178
7.95
A1Z9M2
NP_610936
AAF58285
ReepB-PB
FBpp0291639
18.6
160
8.59
F2FB55
NP_001188926
ADV37172
ReepB-PC
FBpp0291640
17.5
153
6.50
A0A0B4JD51
NP_001188927
ADV37173
ReepB-PD
FBpp0291641
20.3
178
7.95
A1Z9M2
NP_001188928
ADV37174
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

178 aa isoforms: ReepB-PA, ReepB-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ReepB using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.26

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 2
embryo
(O'Neill and Clark, 2013)
embryonic stage 14 -- 16
embryonic/larval salivary gland
(O'Neill and Clark, 2013)
Additional Descriptive Data

ReepB transcripts are detected in the salivary glands of late-stage embryos. Maternal transcripts are also detected in early embryos. The expression pattern was compared to that of other Drosophila species.

(O'Neill and Clark, 2013)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in axon
inferred from direct assay
(Yalçın et al., 2017)
located_in endomembrane system
inferred from high throughput direct assay
(Tan et al., 2009)
located_in endoplasmic reticulum
inferred from direct assay
(Yalçın et al., 2017)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ReepB in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ReepB
Transgenic constructs containing regulatory region of ReepB
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2R)Exel7130
(Ryder, 2004.4.26)
Inferred from location ( 4 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (6)
Hsap\REEP5
14 of 14
Yes
Yes
1  
Hsap\REEP6
12 of 14
No
Yes
1  
Hsap\REEP1
3 of 14
No
No
2  
Hsap\REEP2
3 of 14
No
No
Hsap\REEP3
3 of 14
No
No
Hsap\REEP4
3 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (6)
Rnor\Reep5
14 of 14
Yes
Yes
Rnor\Reep6
12 of 14
No
Yes
Rnor\Reep1
3 of 14
No
No
Rnor\Reep3
3 of 14
No
No
Rnor\Reep4
3 of 14
No
No
Rnor\Reep2
2 of 14
No
No
Mus musculus (laboratory mouse) (6)
Mmus\Reep5
14 of 14
Yes
Yes
Mmus\Reep6
10 of 14
No
Yes
Mmus\Reep2
3 of 14
No
No
Mmus\Reep3
3 of 14
No
No
Mmus\Reep4
3 of 14
No
No
Mmus\Reep1
2 of 14
No
No
Xenopus tropicalis (Western clawed frog) (9)
Xtro\reep5
8 of 13
Yes
Yes
Xtro\nxpe3
6 of 13
No
Yes
Xtro\reep6
6 of 13
No
Yes
Xtro\pitpnc1l
5 of 13
No
Yes
Xtro\reep2
2 of 13
No
No
Xtro\reep4
2 of 13
No
No
Xtro\malt1
1 of 13
No
No
Xtro\reep1
1 of 13
No
No
Xtro\reep3
1 of 13
No
No
Danio rerio (Zebrafish) (7)
Drer\reep5
13 of 14
Yes
Yes
Drer\zgc:101744
12 of 14
No
Yes
Drer\reep6
11 of 14
No
Yes
Drer\reep1
3 of 14
No
No
Drer\reep2
3 of 14
No
No
Drer\reep3a
3 of 14
No
No
Drer\reep3b
3 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (8)
Cele\yop-1
14 of 14
Yes
Yes
Cele\T03F6.6
10 of 14
No
Yes
Cele\F56B3.6
9 of 14
No
Yes
Cele\Y110A2AR.1
4 of 14
No
Yes
Cele\T19C3.4
3 of 14
No
No
Cele\C06E1.1
2 of 14
No
No
Cele\W01D2.3
1 of 14
No
Yes
Cele\Y48B6A.5
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP004819
11 of 12
Yes
Yes
Agam\AgaP_AGAP011331
2 of 12
No
No
Arabidopsis thaliana (thale-cress) (14)
Atha\HVA22B
9 of 13
Yes
Yes
Atha\HVA22D
9 of 13
Yes
Yes
Atha\HVA22E
9 of 13
Yes
Yes
Atha\HVA22F
9 of 13
Yes
Yes
Atha\HVA22A
8 of 13
No
Yes
Atha\HVA22C
8 of 13
No
Yes
Atha\HVA22K
8 of 13
No
Yes
Atha\AT5G42560
3 of 13
No
No
Atha\HVA22G
3 of 13
No
No
Atha\HVA22H
3 of 13
No
No
Atha\HVA22J
3 of 13
No
No
Atha\AT1G04625
1 of 13
No
Yes
Atha\AT1G77815
1 of 13
No
Yes
Atha\AT2G46460
1 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\YOP1
12 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\yop1
12 of 12
Yes
Yes
Spom\SPBC30D10.09c
3 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:ReepB. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (5)
CG4960
12 of 13
Reepl1
6 of 13
CG5539
4 of 13
ReepA
4 of 13
CG30177
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    ReepB48
    model of  hereditary spastic paraplegia
    CEA with ReepA541
    (Oliva et al., 2020)
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    REEP6; receptor accessory protein 6
    model of  retinitis pigmentosa 77
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    REEP5; receptor accessory protein 5
    14 of 14
    125265
         
        REEP6; receptor accessory protein 6
        12 of 14
        609346
         
        REEP1; receptor accessory protein 1
        3 of 14
        RECEPTOR EXPRESSION-ENHANCING PROTEIN 1; REEP1
         
        REEP2; receptor accessory protein 2
        3 of 14
        609347
        REEP3; receptor accessory protein 3
        3 of 14
        609348
            REEP4; receptor accessory protein 4
            3 of 14
            609349
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Interactions
                Summary of Physical Interactions
                Interaction Browsers
                Summary of Genetic Interactions
                Interaction Browsers
                View in FlyBase Interactions Browser   View in MIST tool (external link)
                Please look at the allele data for full details of the genetic interactions
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Rtnl1
                enhanceable
                ReepA|ReepB
                (Yalçın et al., 2017)
                Rtnl1
                enhanceable
                ReepB
                (Yalçın et al., 2017)
                External Data
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                DroID - A comprehensive database of gene and protein interactions.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                FlyBase
                External Links
                External Data
                Linkouts
                Class of Gene
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                2R
                Recombination map
                2-69
                Cytogenetic map
                50E1-50E1
                Sequence location
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                50E1-50E1
                Limits computationally determined from genome sequence between P{lacW}mamk05821 and P{lacW}l(2)s3475s3475&P{lacW}Hsc70-5k04907
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                Experimentally Determined Recombination Data
                Location

                2-69

                (FlyBase, 2016)

                2-74.5

                (Comeron et al., 2012)
                Left of (cM)
                Right of (cM)
                Notes
                Stocks and Reagents
                Stocks (15)
                Genomic Clones (21)
                cDNA Clones (36)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequenced
                BDGP DGC clones
                Other clones
                Drosophila Genomics Resource Center cDNA clones

                For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                Other clones
                RNAi and Array Information
                Linkouts
                DRSC - Results frm RNAi screens
                Antibody Information
                Laboratory Generated Antibodies
                 
                Commercially Available Antibodies
                 
                Cell Line Information
                Publicly Available Cell Lines
                 
                  Other Stable Cell Lines
                   
                    Other Comments
                    Relationship to Other Genes
                    Source for database merge of
                    Additional comments

                    FlyBase curator comment: Both CG8331 and CG4960 show orthology to mammalian REEP5 and REEP6, but CG8331 was designated ReepB because of its widespread expression and slower rate of evolutionary sequence divergence .

                    (Yalçın et al., 2017)

                    The CG4960 gene is a retroduplication of either the CG8331-RA or CG8331-RD transcript, which encode identical proteins.

                    (O'Neill and Clark, 2013)

                    The CG4960 gene may be derived from the CG8331 gene by retroposition.

                    (Betran et al., 2002)
                    Nomenclature History
                    Source for database identify of

                    Source for identity of: ReepB CG8331

                    (Yalçın et al., 2017)
                    Nomenclature comments
                    Etymology

                    The gene is named 'ReepB' due to orthology to two REEP mammalian genes (REEP5 and REEP6).

                    (Yalçın et al., 2017)
                    Synonyms and Secondary IDs (4)
                    Datasets (0)
                    Study focus (0)
                    Experimental Role
                    Project
                    Project Type
                    Title
                    Study result (0)
                    Result
                    Result Type
                    Title
                    External Crossreferences and Linkouts ( 44 )
                    Sequence Crossreferences
                    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                    Other crossreferences
                    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                    FlyMine - An integrated database for Drosophila genomics
                    InterPro - A database of protein families, domains and functional sites
                    KEGG Genes - Molecular building blocks of life in the genomic space.
                    MARRVEL_MODEL - MARRVEL (model organism gene)
                    Linkouts
                    BioGRID - A database of protein and genetic interactions.
                    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                    DroID - A comprehensive database of gene and protein interactions.
                    DRSC - Results frm RNAi screens
                    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                    Flygut - An atlas of the Drosophila adult midgut
                    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    References (54)