FlyBase Gene Report: Dmel\hbt

General Information

Symbol

Dmel\hbt

Species

D. melanogaster

Name

headbutt

Annotation Symbol

CG43778

Feature Type

protein_coding_gene

FlyBase ID

FBgn0264308

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

34B5-34B6

Sequence location

2L:13,301,814..13,331,121 [-]

Recombination map

2-48

RefSeq locus

NT_033779 REGION:13301814..13331121

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in regulation of sensory perception

inferred from mutant phenotype

(Zhang et al., 2020)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in membrane

inferred from biological aspect of ancestor with PANTHER:PTN001387085

(GO Reference Genome Project, 2011-)

located_in membrane

inferred from sequence or structural similarity with FLYBASE:rost; FB:FBgn0011705

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Rolling stone

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\hbt for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A6H2EJZ8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Unconventional translation start (UUG) postulated; FlyBase analysis.

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.53

Stop-codon suppression (UAA) postulated; FBrf0243886.

Gene model reviewed during 6.32

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

hbt-RA

FBtr0331503

NM_001273513

6433

963

hbt-RC

FBtr0331504

NM_135793

2595

663

hbt-RD

FBtr0331505

NM_135791

6433

451

hbt-RE

FBtr0479944

NM_001382118

6433

1003

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

hbt-PA

FBpp0303893

106.8

963

6.76

FBpp0303894

75.5

663

6.43

FBpp0303895

49.6

451

8.46

FBpp0428276

111.6

1003

6.94

QJC20911

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Rolling stone

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\hbt using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG43778_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.73

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\hbt in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\hbt

View exonic expression by tissue for Dmel\hbt

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34738/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264308

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032504

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264308

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of hbt

Transgenic constructs containing regulatory region of hbt

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

hbt^GD1119

viable, with Scer\GAL4^Mef2.PR

hbt^GD1119

viable, with Scer\GAL4^pnr-MD237

Other Phenotypes

Allele

abnormal touch response | larval stage

abnormal touch response | larval stage, with Scer\GAL4^ChAT.PU

hbt^RNAi.UAS.cUa

abnormal touch response | larval stage (with hbt^KO)

hbt^MI03974

abnormal touch response | larval stage (with hbt^MI03974)

hbt^KO

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (1)

Drer\LOC103911277

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP009583

2 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:hbt. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (5)

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264308

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

gene_with_non_canonical_start_codon

gene_with_stop_codon_read_through

(FlyBase Genome Annotators, 2020)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-48

Cytogenetic map

34B5-34B6

Sequence location

2L:13,301,814..13,331,121 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34B5-34B6

Limits computationally determined from genome sequence between P{EP}EP850&P{lacW}l(2)k05911^k05911 and P{lacW}l(2)k07914^k07914&P{lacW}Nnp-1^k07826;

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-48

(FlyBase, 2016)

2-43.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

40198

y¹ w^*; Mi{MIC}hbt^MI03974

40778

y¹ w^*; Mi{MIC}hbt^MI05240/SM6a

NIG-Fly

16970R-3

P{NIG.16970R}3

M2L-1037

y¹ w¹¹¹⁸; hbt^SK1 P{neoFRT}40A/CyO-Tb

M2L-1038

y¹ w¹¹¹⁸; hbt^SK7 P{neoFRT}40A/CyO-Tb

2LG-0832

y² VhaAC39-1^cho-1 v¹; P{U6-hbt.NIG.sgRNA}attP40/CyO

VDRC

v7699

w¹¹¹⁸; P{GD1119}v7699

Genomic Clones (30)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (3)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

IP20291

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0264308

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Annotation CG15639 split in release 5.45 of the genome annotation. The 5' exon of CG15639 has been made into a distinct annotation, CG43779. The remainder of CG15639 has been merged with the CG16970 annotation, generating the CG43778 annotation.

(FlyBase Genome Annotators, 2012)

Merge supported by second tier modENCODE RNA-seq junction data (FBrf0217580) and RNA-seq expression data.

(FlyBase Genome Annotators, 2012)

Nomenclature History

Source for database identify of

Source for identity of: hbt CG43778

(Zhang et al., 2020)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG15639

(Smibert et al., 2012, Jungreis, 2011.11.18)

CG16970

(Izutsu et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Rodriguez et al., 2012)

CG43778

(National Institute of Genetics Fly Stocks, 2022-, FlyBase Genome Annotators, 2020, Zhang et al., 2020, FlyBase Genome Annotators, 2019-, FlyBase Genome Annotators, 2012)

FBgn 32504

(Lehmann et al., 2010)

hbt

(Zhang et al., 2020)

Name Synonyms

headbutt

(Zhang et al., 2020)

Secondary FlyBase IDs

FBgn0264300
FBgn0032504
FBgn0032502

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 30 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34738

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A6H2EJZ8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A6H2EJZ8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34738/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264308

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264308

InterPro - A database of protein families, domains and functional sites

Rolling stone

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG43778

MARRVEL_MODEL - MARRVEL (model organism gene)

34738

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0264308

DroID - A comprehensive database of gene and protein interactions.

FBgn0264308

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG43778_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032504

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0264308

References (29)

The following external sites may use different assemblies or annotations than FlyBase.

-0.73

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