In an effort to identify genes critical to human heart development that are also expressed in other tissues, a screen for genes with cardiac-specific cis-regulatory elements was conducted in human. Identified candidate genes were assessed using orthologous genes in animal models, including Drosophila. The human PSMD7 gene, which encodes a component of the 26S proteasome, was identified in this screen; the orthololgous Drosophila gene is Rpn8. Multiple genetic reagents have been generated for Dmel\Rpn8 including RNAi-targeting constructs, overexpression constructs, and alleles caused by insertional mutagenesis.
The human PSMD7 gene has not been introduced into flies.
Knockdown of Dmel\Rpn8 specifically in developing cardiac cells, effected by RNAi, results in detectable heart development defects in larvae and 96% lethality.
[updated Feb. 2022 by FlyBase; FBrf0222196]
PSMD7 encodes a component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. [Gene Cards, PSMD7; 2022.02.06]
One to one: 1 human gene to 1 Drosophila gene.
High-scoring ortholog of human PSMD7 (1 Drosophila to 1 human).
