FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mio, Nup44A
FlyBase ID
FBig0000023370
Interaction Network
Interactions Browser links
mio network
Nup44A network
Reported Interactions
FBrf0213566-1.coIP.MS
Reference
Senger et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mio 

Mio

bait

FLAG tag, HA tag

Nup44A 

Seh1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

mio protein was purified by FLAG HA tandem affinity purification.

(Senger et al., 2011)

mio interacts with Nup44A, but not other nucleoporin proteins tested (Nup107, Mtor).

(Senger et al., 2011)

Bait expressed in transfected cells.

(Senger et al., 2011)

Prey endogenous to cell extract.

(Senger et al., 2011)
FBrf0213566-2.coIP.WB
Reference
Senger et al., 2011
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mio 

Mio

bait
Nup44A 

Seh1

prey

CeFP tag, HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Protein extract was made from dissected ovary of transgenic females.

(Senger et al., 2011)

CeFP tag recognized by GFP antibody.

(Senger et al., 2011)

Prey derived from a transgenic line carrying CeFP- and HA-tagged UAS-Nup44A construct, P{UASp-Nup44A.CeFP.HA}.

(Senger et al., 2011)

Bait endogenous to ovary extract.

(Senger et al., 2011)
FBrf0218395-7369.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mio 
unspecified role
mio 
unspecified role
mio 
unspecified role
Nup44A 
unspecified role
Nup44A 
unspecified role
Nup44A 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 61.62630

FBrf0232362-4.coIP.MS
Reference
Cai et al., 2016
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mio 

Mio

bait

HA tag, FLAG tag

Nup44A 

Seh1

prey

20 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

FLAG-HA tandem affinity purification.

(Cai et al., 2016)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Cai et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)