Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system.

Components of the "WRC" complex were co-expressed in Sf9 cells (HSPC300, Sra-1, Abi, SCAR, Hem), pulled down via GST tag, and further purified by size exclusion column.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system.

Components of the "WRC" complex were co-expressed in Sf9 cells (HSPC300, Sra-1, Abi, SCAR, Hem), pulled down via MBP tag, and further purified by size exclusion column.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Purified WAVE regulatory complex (WRC).

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Pull down was carried out with all proteins WRC complex (Hem, SCAR, Abi, Cyfip, and HSPC300). WRC complex captured as four pairwise interactions with HSPC300 as bait.

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.