Interaction in vitro; bait derived from wild type embryonic nuclear extract; prey produced and labeled by in vitro translation.
Purified Brahma complex was resolved by SDS-PAGE, transferred to a blot and proteins were renatured. The blot was then incubated with radiolabeled prey protein.
Source was embryos of wild-type fly line; proteins produced from endogenous genes.
HGScore = 377.50498
BAP complex purified via d4-HA immunoprecipitation, cross-links detected by mass spectrometry.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
