FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
psidin, Naa20A
FlyBase ID
FBig0000050230
Interaction Network
Interactions Browser links
psidin network
Naa20A network
Reported Interactions
FBrf0218395-1415.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Naa20A 
unspecified role
Naa20A 
unspecified role
Naa20A 
unspecified role
psidin 
unspecified role
psidin 
unspecified role
psidin 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 135.59771

FBrf0220017-1.coIP.WB
Reference
Stephan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
psidin 

Psidin

bait

HA tag

psidin 
psidin 
Naa20A 

dNAA20

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
psidin 
NatB interaction domain
necessary binding region
aa 264-645
psidin 
phosphorylation site
mutation disrupting interaction
aa 678

S678D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Stephan et al., 2012)

The S678D mutation of psidin, mimicking the phosphorylated state, inhibits its interaction with NAA20. The S678A mutation, which mimics the unphosphorylated state, does not disrupt the interaction.

(Stephan et al., 2012)
FBrf0247133-1.coIP.WB
Reference
Lasa et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Naa20A 

dNAA20

bait

Myc tag

Naa20A 
psidin 

dNAA25

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Naa20A 
interaction domain residue 2
mutation decreasing interaction
aa 2

T2I, T2D, T2F or T2P, fcoordinates relative to Naa20A-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK-293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lasa et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)