FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
psq, Trl
FlyBase ID
FBig0000062924
Interaction Network
Interactions Browser links
psq network
Trl network
Reported Interactions
FBrf0215054-19.Y2H
Reference
Bonchuk et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAF

bait

fused to GAL4 DNA-binding domain

Trl 
psq 

Pipsqueak

prey

fused to GAL4 activation domain

psq 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
BTB domain
sufficient binding region
aa 1-131
psq 
BTB domain
sufficient binding region
aa 1-122
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2011)
FBrf0215054-27.Y2H
Reference
Bonchuk et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
psq 

Pipsqueak

bait

fused to GAL4 DNA-binding domain

psq 
Trl 

GAF

prey

fused to GAL4 activation domain

Trl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
BTB domain
sufficient binding region
aa 1-131
psq 
BTB domain
sufficient binding region
aa 1-122
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2011)
FBrf0218395-532.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
psq 
unspecified role
psq 
unspecified role
psq 
unspecified role
Trl 
unspecified role
Trl 
unspecified role
Trl 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 88.34854

FBrf0253740-15.coIP.WB
Reference
Chetverina et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAF

bait
psq 

Psq

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Chetverina et al., 2022)
FBrf0253740-32.coIP.MS
Reference
Chetverina et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
psq 

Psq

bait
Trl 

GAF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Chetverina et al., 2022)
FBrf0253740-6.coIP.WB
Reference
Chetverina et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
psq 

Psq

bait
Trl 

GAF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Chetverina et al., 2022)
FBrf0260563-20.Y2H
Reference
Bonchuk et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
psq 

psq

bait

fused to GAL4 DNA-binding domain

psq 
Trl 

GAF

prey

fused to GAL4 activation domain

Trl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
TTK-type BTB domain
sufficient binding region
aa 1-125
psq 
TTK-type BTB domain
sufficient binding region
aa 1-127
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2024)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2024)
FBrf0260563-21.Y2H
Reference
Bonchuk et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAF

bait

fused to GAL4 DNA-binding domain

Trl 
psq 

psq

prey

fused to GAL4 activation domain

psq 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
TTK-type BTB domain
sufficient binding region
aa 1-125
psq 
TTK-type BTB domain
sufficient binding region
aa 1-127
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2024)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)