FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
eIF4EHP, Gyf
FlyBase ID
FBig0000073627
Interaction Network
Interactions Browser links
eIF4EHP network
Gyf network
Reported Interactions
FBrf0218395-6485.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gyf 
unspecified role
Gyf 
unspecified role
Gyf 
unspecified role
eIF4EHP 
unspecified role
eIF4EHP 
unspecified role
eIF4EHP 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 107.23189

FBrf0243046-1.coIP.WB
Reference
Ruscica et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
eIF4EHP 

4EHP

bait

GFP tag

eIF4EHP 
eIF4EHP 
eIF4EHP 
Gyf 

GIGYF

prey

HA tag

Gyf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gyf 
N-terminal domain
sufficient binding region
aa 1-640

coordinates relative to Gyf-PG

eIF4EHP 
dorsal surface residue 85
mutation disrupting interaction
aa 85

W85A, coordinates relative to eIF4EHP-PA

eIF4EHP 
lateral surface residues 75,91
mutation disrupting interaction
aa 75,91

V75A,L91A, coordinates relative to eIF4EHP-PA

eIF4EHP 
specific surface residues 93,139
mutation disrupting interaction
aa 93,139

R93P,E139L, coordinates relative to eIF4EHP-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ruscica et al., 2019)
FBrf0243046-13.coIP.WB
Reference
Ruscica et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Gyf 

GIGYF

bait

HA or GFP tag

Gyf 
eIF4EHP 

4EHP

prey

GFP or HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gyf 
dorsal surface residues 58,60,65
mutation disrupting interaction
aa 58,60,65

Y58A,Y60A,M65A, coordinates relative to Gyf-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ruscica et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)