FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
egg, wde
FlyBase ID
FBig0000074794
Interaction Network
Interactions Browser links
egg network
wde network
Reported Interactions
FBrf0208842-1.coIP.WB
Reference
Koch et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
wde 

Wde

bait

GFP tag

egg 

Egg

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged wde and egg constructs.

(Koch et al., 2009)

Even though GFP-tagged wde construct used, anti-wde antibody used for IP.

(Koch et al., 2009)
FBrf0208842-2.coIP.WB
Reference
Koch et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
wde 

Wde

bait

GFP tag

egg 

Egg

prey

HA tag

egg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egg 
region
unspecified role
aa 366–521
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged wde and egg constructs.

(Koch et al., 2009)
FBrf0208842-3.coIP.WB
Reference
Koch et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
egg 

Egg

bait

HA tag

wde 

Wde

prey

GFP tag

wde 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
wde 
coiled-coil domain
unspecified role
aa 842–907
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged wde and egg constructs.

(Koch et al., 2009)
FBrf0218395-457.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wde 
unspecified role
wde 
unspecified role
wde 
unspecified role
egg 
unspecified role
egg 
unspecified role
egg 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 74.01168

FBrf0241908-1.coIP.WB
Reference
Seller et al., 2019
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egg 

Egg

bait

GFP tag

wde 

Wde

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Seller et al., 2019)
FBrf0244218-1.coIP.WB
Reference
Osumi et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
wde 

Wde

bait

FLAG tag

wde 
egg 

Egg

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
wde 
coiled-coil domain
necessary binding region
aa 858-885

coordinates relative to wde-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from transfected construct.

(Osumi et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)