FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Gαo, Rab5
FlyBase ID
FBig0000077762
Interaction Network
Interactions Browser links
Gαo network
Rab5 network
Reported Interactions
FBrf0211592-1.PD.WB
Reference
Purvanov et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab5 

Rab5

bait

GST pull down

Gαo 

Gα[[o]]

prey

His6 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Showed binding of Rab5-GDP at higher level than Rab5-GTP.

(Purvanov et al., 2010)

Cloned reagents; interaction in solution.

(Purvanov et al., 2010)
FBrf0211592-3.PD.WB
Reference
Purvanov et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gαo 

Gα[[o]]

bait
Rab5 

Rab5

prey

His6 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Showed binding of Rab5-GDP at higher level than Rab5-GTP.

(Purvanov et al., 2010)

Showed no significant difference between binding of G-oα47A-GDP and G-oα47A-GTP.

(Purvanov et al., 2010)

Cloned reagents; interaction in solution.

(Purvanov et al., 2010)
FBrf0211592-4.PD.WB
Reference
Purvanov et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab5 

Rab5

bait
Gαo 

Gα[[o]]

prey

His6 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cloned reagents; interaction in solution.

(Purvanov et al., 2010)
FBrf0211592-5.PD.WB
Reference
Purvanov et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gαo 

Gα[[o]]

bait
Rab5 

Rab5

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey derived from transgenic line carrying GAL4/UAS-Rab5 constructs.

(Purvanov et al., 2010)
FBrf0211592-6.PD.WB
Reference
Purvanov et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab5 

Rab5

bait
Gαo 

Gα[[o]]

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey derived from transgenic line carrying GAL4/UAS-G-oα47A constructs.

(Purvanov et al., 2010)

Showed binding of Rab5-GDP at higher level than Rab5-GTP.

(Purvanov et al., 2010)
FBrf0211592-7.PD.WB
Reference
Purvanov et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab5 

Rab5

bait
Gαo 

Gα[[o]]

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

wild-type

(Purvanov et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)