FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rcd5, MBD-R2
FlyBase ID
FBig0000085806
Interaction Network
Interactions Browser links
Rcd5 network
MBD-R2 network
Reported Interactions
FBrf0211222-15.coIP.MS
Reference
Raja et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rcd5 

MCRS2

bait

TAP tag

MBD-R2 

MBDR2

prey

10 peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Raja et al., 2010)
FBrf0211222-24.coIP.WB
Reference
Raja et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rcd5 

MCRS2

bait
MBD-R2 

MBDR2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Raja et al., 2010)
FBrf0218395-3602.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rcd5 
unspecified role
Rcd5 
unspecified role
Rcd5 
unspecified role
MBD-R2 
unspecified role
MBD-R2 
unspecified role
MBD-R2 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 61.66572

FBrf0241907-10.coIP.WB
Reference
Lam et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
MBD-R2 

MBD-R2

bait

FLAG tag

Rcd5 

MCRS2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct or endogenous gene; prey produced from endogenous gene.

(Lam et al., 2019)
FBrf0241907-19.coIP.WB
Reference
Lam et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rcd5 

MCRS2

bait
MBD-R2 

MBD-R2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lam et al., 2019)
FBrf0264203-26.CH.WB
Reference
Lenz et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MBD-R2 

MBD-R2

neutral component
MBD-R2 

MBD-R2

neutral component
MBD-R2 

MBD-R2

neutral component
Rcd5 

Rcd5

neutral component
Rcd5 

Rcd5

neutral component
Rcd5 

Rcd5

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

hang, nsl1, MBD-R2, Rcd5 and mof form a complex, captured as pairwise interactions.

(Lenz et al., 2025)

Interaction in vitro; proteins produced from endogenous gene.

(Lenz et al., 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)