FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Rcd5
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General Information
Symbol
Dmel\Rcd5
Species
D. melanogaster
Name
Reduction in Cnn dots 5
Annotation Symbol
CG1135
Feature Type
protein_coding_gene
FlyBase ID
FBgn0263832
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
Reduction in Cnn dots 5 (Rcd5) encodes a protein that belongs to the family of forkhead-associated domain proteins. As part of the non-specific lethal complex, it regulates transcription of housekeeping genes by facilitating recruitment of RNA polymerase II to promoters. During mitosis it plays a key role in chromosomal-dependent microtubule assembly, centriole duplication and pericentriolar material maturation. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

MCRS2, MCRS1, dMCRS2

Key Links
Genomic Location
Cytogenetic map
64A4-64A4
Sequence location
Recombination map
3-10
RefSeq locus
NT_037436 REGION:4040529..4042597
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with FLYBASE:nsl1; FB:FBgn0262527
(Raja et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables G-quadruplex RNA binding
inferred from electronic annotation with InterPro:IPR037912
(InterPro Project Members, 2004-)
Biological Process (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in centriole replication
inferred from mutant phenotype
(Dobbelaere et al., 2008)
involved_in positive regulation of transcription by RNA polymerase II
inferred from mutant phenotype
(Raja et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in positive regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN000325907
(GO Reference Genome Project, 2011-)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
part_of NSL complex
inferred from direct assay
(Raja et al., 2010)
located_in nucleus
inferred from direct assay
(Raja et al., 2010, Dobbelaere et al., 2008, Wagner et al., 2007)
located_in polytene chromosome interband
inferred from direct assay
(Raja et al., 2010)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
part_of Ino80 complex
inferred from electronic annotation with InterPro:IPR037912
(InterPro Project Members, 2004-)
part_of MLL1 complex
inferred from electronic annotation with InterPro:IPR037912
(InterPro Project Members, 2004-)
part_of NSL complex
inferred from biological aspect of ancestor with PANTHER:PTN000325907
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Reduction in Cnn dots 5 (Rcd5) encodes a protein that belongs to the family of forkhead-associated domain proteins. As part of the non-specific lethal complex, it regulates transcription of housekeeping genes by facilitating recruitment of RNA polymerase II to promoters. During mitosis it plays a key role in chromosomal-dependent microtubule assembly, centriole duplication and pericentriolar material maturation. [Date last reviewed: 2019-03-14]
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Rcd5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VZJ3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Rcd5-RA
FBtr0073202
NM_139595
1947
578
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Rcd5-PA
FBpp0073058
63.5
578
9.94
Q9VZJ3
NP_647852
AAF47828
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rcd5 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.02

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of NSL complex
inferred from direct assay
(Raja et al., 2010)
located_in nucleus
inferred from direct assay
(Raja et al., 2010, Dobbelaere et al., 2008, Wagner et al., 2007)
located_in polytene chromosome interband
inferred from direct assay
(Raja et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Rcd5 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rcd5
Transgenic constructs containing regulatory region of Rcd5
Aberrations (Deficiencies and Duplications) ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (3)
Hsap\MCRS1
14 of 14
Yes
Yes
Hsap\FOXK1
1 of 14
No
No
Hsap\FOXK2
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Mcrs1
13 of 14
Yes
Yes
Mus musculus (laboratory mouse) (3)
Mmus\Mcrs1
13 of 14
Yes
Yes
Mmus\Foxk1
1 of 14
No
No
Mmus\Foxk2
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (2)
Xtro\mcrs1
7 of 13
Yes
Yes
Xtro\znf519
5 of 13
No
Yes
Danio rerio (Zebrafish) (4)
Drer\mcrs1
14 of 14
Yes
Yes
Drer\foxk1
1 of 14
No
No
Drer\foxk2a
1 of 14
No
No
Drer\foxk2b
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\mcrs-1
12 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP005560
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (5)
Atha\AT1G75530
9 of 13
Yes
Yes
Atha\emb1967
7 of 13
No
Yes
Atha\AT1G60700
5 of 13
No
Yes
Atha\AT3G07260
1 of 13
No
Yes
Atha\FHA2
1 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\FHL1
1 of 13
Yes
No
Scer\FKH1
1 of 13
Yes
No
Scer\FKH2
1 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\fhl1
1 of 12
Yes
No
Spom\fkh2
1 of 12
Yes
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Rcd5. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MCRS1; microspherule protein 1
    14 of 14
    609504
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Rcd5 - Caf1-55
        anti bait coimmunoprecipitation, western blot
        (Lam et al., 2019)
        Rcd5 - E(bx)
        anti bait coimmunoprecipitation, western blot
        (Lam et al., 2019)
        Rcd5 - Ino80
        anti bait coimmunoprecipitation, western blot
        (Lam et al., 2019)
        Rcd5 - Iswi
        anti bait coimmunoprecipitation, western blot
        (Lam et al., 2019)
        Rcd5 - MBD-R2
        anti bait coimmunoprecipitation, western blot, coimmunoprecipitation, molecular sieving, anti tag coimmunoprecipitation, Identification by mass spectrometry, experimental knowledge based
        (Lenz et al., 2025, Lam et al., 2019, Guruharsha et al., 2011, Raja et al., 2010)
        Rcd5 - Nurf-38
        anti bait coimmunoprecipitation, western blot
        (Lam et al., 2019)
        Rcd5 - Pitslre
        tandem affinity purification, Identification by mass spectrometry, cosedimentation, western blot, anti bait coimmunoprecipitation
        (Andersen et al., 2010)
        Rcd5 - Polr2A
        cosedimentation, western blot, anti bait coimmunoprecipitation
        (Andersen et al., 2010)
        Rcd5 - Rcd1
        anti tag coimmunoprecipitation, Identification by mass spectrometry, pull down, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot
        (Lam et al., 2019, Meunier et al., 2015, Raja et al., 2010)
        Rcd5 - dgt1
        anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot, pull down, molecular weight estimation by staining
        (Lam et al., 2019, Dias et al., 2014, Raja et al., 2010)
        Rcd5 - hang
        anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot, molecular sieving
        (Lenz et al., 2025)
        Rcd5 - mof
        anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry, molecular sieving
        (Lenz et al., 2025, Lam et al., 2019, Raja et al., 2010, Mendjan et al., 2006)
        Rcd5 - nsl1
        pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry, molecular sieving
        (Lenz et al., 2025, Lam et al., 2019, Dias et al., 2014, Raja et al., 2010)
        Rcd5 - wds
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Raja et al., 2010)
        Summary of Genetic Interactions
        Interaction Browsers
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3L
        Recombination map
        3-10
        Cytogenetic map
        64A4-64A4
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        64A4-64A4
        Limits computationally determined from genome sequence between P{PZ}Sc205634 and P{PZ}l(3)rG166rG166
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        64A4-64A5
        (determined by in situ hybridisation)
        (Spradling et al., 1999)
        64A4-64A5
        (BDGP Project Members, 1994-1999)
        Experimentally Determined Recombination Data
        Location

        3-10

        (FlyBase, 2016)

        3-8.6

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (7)
        Genomic Clones (22)
        cDNA Clones (56)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            Rcd5 is involved in centriole duplication and is required for efficient recruitment of pericentriolar material.

            (Dobbelaere et al., 2008)

            RNAi screen using dsRNA made from templates generated with primers directed against this gene results in decreased γ-tubulin staining of the centrosome and spindle and the formation of an aberrantly long spindle when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype is not observed without Cdc27 dsRNA.

            (Goshima et al., 2007)

            Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.

            (Nybakken et al., 2005)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: Rcd5 l(3)rG166

            (Andersen et al., 2010)
            Additional comments
            Nomenclature History
            Source for database identify of

            Source for identity of: Rcd5 CG1135

            (Dobbelaere et al., 2008)
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (12)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 39 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
            References (65)