FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pbl, tum
FlyBase ID
FBig0000086109
Interaction Network
Interactions Browser links
pbl network
tum network
Reported Interactions
FBrf0158856-1.Y2H
Reference
Somers and Saint, 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 9blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic cycle 3embryonic stage 4embryonic cycle 9embryonic stage 2embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pbl 

PBL

bait
tum 

RacGAP50C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey isolated from embryonic cDNA library.

(Somers and Saint, 2003)

Two-hybrid system: yeast LexA/VP16.

(Somers and Saint, 2003)
FBrf0158856-2.Y2H
Reference
Somers and Saint, 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pbl 

PBL

bait
pbl 
pbl 
pbl 
tum 

RacGAP50C

prey
tum 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pbl 
N-terminal region
unspecified role
aa 1-206

coordinates refer to pbl-PA

pbl 
Rad4/Ect2-like domain
unspecified role

within N-terminal region

pbl 
BRCA1 C-terminal domain
unspecified role

within N-terminal region

tum 
coiled coil domain
unspecified role
aa 65-104
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey was cloned reagent (previously isolated).

(Somers and Saint, 2003)

Two-hybrid system: yeast LexA/VP16.

(Somers and Saint, 2003)
FBrf0158856-3.coIP.WB
Reference
Somers and Saint, 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 10blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic stage 9embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pbl 

PBL

bait
tum 

RacGAP50C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0214097-4.Y2H
Reference
Ebrahimi et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pbl 

Pebble

bait

fused to GAL4 DNA-binding domain

tum 

RacGAP

prey

fused to VP16 activation domain

tum 
tum 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tum 
Polo kinase binding region
mutation decreasing interaction
aa 119-122

multiple alanine substitutions

tum 
Polo kinase binding region
mutation disrupting interaction
aa 154-155

double alanine substitution

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ebrahimi et al., 2010)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Ebrahimi et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)