FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
scra, tum
FlyBase ID
FBig0000086223
Interaction Network
Interactions Browser links
scra network
tum network
Reported Interactions
FBrf0203072-1.Y2H
Reference
Gregory et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
scra 

Anillin

bait

fused to LexA DNA-binding domain

scra 
tum 

RacGAP

prey

fused to VP16 activation domain

tum 
tum 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tum 
interaction domain
sufficient binding region
aa 83-309
tum 
interaction domain
necessary binding region
aa 245-311
scra 
C terminal region
sufficient binding region
aa 517-1212
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Gregory et al., 2008)

The interaction is unaffected by removal of the Pebble- or Pav-KLP-binding domains in tum. The C1 and GAP domains in tum and the N-terminal half of scra did not show any interactions.

(Gregory et al., 2008)

Two-hybrid system: yeast LexA-BD/VP16-AD

(Gregory et al., 2008)
FBrf0204118-13.coIP.MS
Reference
D'Avino et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
scra 

Anillin

bait

Protein A tag

tum 

RacGAP50C

prey

30 peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(D'Avino et al., 2008)

Cells were treated with the proteasome inhibitor MG132 to increase the mitotic index of cells and enrich for protein complexes involved in cytokinesis.

(D'Avino et al., 2008)
FBrf0204118-18.PD.A
Reference
D'Avino et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
scra 

Anillin

bait

GST tag

scra 
tum 

RacGAP50C

prey

[35]S label

tum 
tum 
tum 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
scra 
Anillin Homology and PH domains
sufficient binding region
aa 929-1239
tum 
central region
sufficient binding region
aa 136-371
tum 
central region
necessary binding region
aa 243-371
tum 
central region
mutation decreasing interaction
aa 136-205

reduced binding

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(D'Avino et al., 2008)
FBrf0211056-2.Y2H
Reference
Jones et al., 2010
Description
physical association
Assay
Collection
Source/Stage
/ embryonic stage 1embryonic stage 9blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic cycle 3embryonic stage 4embryonic cycle 9embryonic stage 2embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tum 

Tum

bait
scra 

Anillin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey isolated from embryonic cDNA library.

(Jones et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Jones et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)