FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO3, aub
FlyBase ID
FBig0000086611
Interaction Network
Interactions Browser links
AGO3 network
aub network
Reported Interactions
FBrf0209532-12.CS.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO3 

AGO3

neutral component
AGO3 

AGO3

neutral component
AGO3 

AGO3

neutral component
aub 

Aub

neutral component
aub 

Aub

neutral component
aub 

Aub

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nishida et al., 2009)
FBrf0209532-13.coIP.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait
AGO3 

AGO3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nishida et al., 2009)
FBrf0209532-6.PD.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO3 

AGO3

bait

biotin tag

AGO3 
aub 

Aub

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO3 
interaction domain
sufficient binding region
aa 58-81

coordinates relative to AGO3-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide commercially synthesized; prey derived from wild-type ovary extract.

(Nishida et al., 2009)
FBrf0209532-7.PD.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

biotin tag

aub 
AGO3 

AGO3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aub 
N-terminal domain
sufficient binding region
aa 1-25

coordinates relative to aub-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide commercially synthesized; prey derived from wild-type ovary extract.

(Nishida et al., 2009)
FBrf0209532-8.coIP.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO3 

AGO3

bait
aub 

Aub

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nishida et al., 2009)
FBrf0212183-3.coIP.WB
Reference
Rouget et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

aub

bait

GFP tag

AGO3 

AGO3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type or transgenic fly line; bait produced from endogenous gene or tagged transgenic construct; prey produced from endogenous gene.

(Rouget et al., 2010)

Interaction also detected in osk mutant background.

(Rouget et al., 2010)
FBrf0216809-1.coIP.WB
Reference
Zhang et al., 2011
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait
AGO3 

Ago3

prey

FLAG-Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Recovery of tagged AGO3 protein reduced 6-fold in lysate from qin mutant ovaries.

(Zhang et al., 2011)

No change with treatment of lystate with RNase A prior to IP, indicating not mediated by RNA.

(Zhang et al., 2011)
FBrf0243392-1.coIP.WB
Reference
Cappucci et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO3 

AGO3

bait
aub 

Aub

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Cappucci et al., 2019)

The AGO3-aub interaction was detected with and without treating flies with heat-shock.

(Cappucci et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)