FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
jvl, spn-F
FlyBase ID
FBig0000087917
Interaction Network
Interactions Browser links
jvl network
spn-F network
Reported Interactions
FBrf0216406-1.Y2H
Reference
Dubin-Bar et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
spn-F 

Spn-F

bait
jvl 

Jvl

prey
jvl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
jvl 
region
unspecified role
aa 400-800
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey was cloned reagent (previously isolated).

(Dubin-Bar et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD; low throughput.

(Dubin-Bar et al., 2011)
FBrf0216406-2.PD.WB
Reference
Dubin-Bar et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
spn-F 

Spn-F

bait

GST pull down

jvl 

Jvl

prey

His tag

jvl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
jvl 
region
unspecified role
aa 400-800
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey was cloned reagent; expressed in bacterial cells.

(Dubin-Bar et al., 2011)
FBrf0223108-2.Y2H
Reference
Amsalem et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
jvl 

Jvl

bait

fused to GAL4 DNA-binding domain

spn-F 

Spn-F

prey

fused to GAL4 activation domain

spn-F 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spn-F 
C-terminal region
sufficient binding region
aa 270-364

\'CT\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Amsalem et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Amsalem et al., 2013)
FBrf0223108-3.coIP.WB
Reference
Amsalem et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
jvl 

Jvl

bait

GFP tag

spn-F 

Spn-F

prey

mCherry tag

spn-F 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spn-F 
C-terminal region
sufficient binding region
aa 270-364

\'CT\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Amsalem et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)