FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000088633
Interaction Network
Interactions Browser links
fln network
Mhc network
Reported Interactions
FBrf0158767-1.FB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mhc 

MHC

bait
Mhc 
Mhc 
Mhc 
fln 

rFln

prey

HIS tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mhc 
myosin rod
mutation disrupting interaction
aa 1554

E1554K

Mhc 
C600
sufficient binding region
aa 1346-1935

C600 construct

Mhc 
LMM
necessary binding region
aa 1346-1540
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; prey produced as a recombinant fusion protein in Sf9 (Spodoptera frugiperda) cell system; bait produced as a recombinant fusion protein in bacterial system.

FBrf0158767-2.PD
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fln 

rFLN

bait

HIS tag

Mhc 

MHC

prey
Mhc 
Mhc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mhc 
rod region
sufficient binding region

MHC for this experiment was obtained from the MHC y57 allele and is missing most of the head region of the MHC molecule.

Mhc 
myosin rod
mutation disrupting interaction
aa 1554

E1554K

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in Sf9 (Spodoptera frugiperda) cell system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0218395-8873.DPiM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fln 
unspecified role
fln 
unspecified role
fln 
unspecified role
Mhc 
unspecified role
Mhc 
unspecified role
Mhc 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 166.14911

FBrf0249365-1.CS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mhc 

MHC

neutral component
Mhc 

MHC

neutral component
Mhc 
Mhc 

MHC

neutral component
Mhc 
fln 

Flightin

neutral component
fln 
fln 

Flightin

neutral component
fln 

Flightin

neutral component
fln 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mhc 
LMM domain
sufficient binding region
aa 1346-1941

coordinates relative to Mhc-PA

fln 
WYR domain
sufficient binding region
aa 84-136
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; Mhc LMM domain produced as a recombinant fusion protein in bacterial system; fln WYR domain peptide commercially synthesized.

Kd = 2.06uM

FBrf0249365-2.CD
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mhc 

MHC

neutral component
Mhc 

MHC

neutral component
Mhc 
Mhc 

MHC

neutral component
Mhc 
fln 

Flightin

neutral component
fln 
fln 

Flightin

neutral component
fln 

Flightin

neutral component
fln 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mhc 
LMM domain
sufficient binding region
aa 1346-1941

coordinates relative to Mhc-PA

fln 
WYR domain
sufficient binding region
aa 84-136
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; Mhc LMM domain produced as a recombinant fusion protein in bacterial system; fln WYR domain peptide commercially synthesized.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)