FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000089250
Interaction Network
Interactions Browser links
Mcm5 network
Mcm2 network
Reported Interactions
FBrf0129950-5.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mcm5 

MCM5

bait
Mcm2 

MCM2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Mcm6, Orc2, and Orc5 were not detected in the immunoprecipitates.

FBrf0193013-31.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mcm5 

Mcm5

bait
Mcm2 

Mcm2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0214314-3.CS.EM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mcm2 

Mcm2

neutral component
Mcm2 

Mcm2

neutral component
Mcm2 

Mcm2

neutral component
Mcm5 

Mcm5

neutral component
Mcm5 

Mcm5

neutral component
Mcm5 

Mcm5

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced in a baculovirus and insect cell system.

The CMG complex is captured as 55 pairwise direct or indirect interactions.

FBrf0218395-7521.DPiM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mcm2 
unspecified role
Mcm2 
unspecified role
Mcm2 
unspecified role
Mcm5 
unspecified role
Mcm5 
unspecified role
Mcm5 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 65.83666

FBrf0228822-9.EM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mcm2 

Mcm2

neutral component
Mcm2 

Mcm2

neutral component
Mcm2 

Mcm2

neutral component
Mcm5 

Mcm5

neutral component
Mcm5 

Mcm5

neutral component
Mcm5 

Mcm5

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced in baculovirus and insect cell system.

Paper reports structure of large complex. Interactions of neighboring proteins curated in pairwise fashion.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)