HGScore = 65.11922
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.
HGScore = 15.253053
Hexameric CCR4-NOT-mini complex captured as 15 pairwise interactions.
Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.
Rga was fused to a plasma membrane or ER membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.
Source was live S2R+ cells; bait and prey produced from endogenous genes.
Source was live S2R+ cells; bait and prey produced from endogenous genes.
Not3 was fused to a plasma membrane or ER membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.
Split-ubiquitin-based membrane yeast two-hybrid (MYTH) assay
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.
Split-ubiquitin-based membrane yeast two-hybrid (MYTH) assay
