FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nup358, sbr
FlyBase ID
FBig0000091746
Interaction Network
Interactions Browser links
Nup358 network
sbr network
Reported Interactions
FBrf0194866-1.coIP.WB
Reference
Kozlova et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
sbr 

NXF1

bait

GFP tag

sbr 
Nup358 

Nup358

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sbr 
NPC-binding domain
sufficient binding region
aa 371-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogeous gene.

(Kozlova et al., 2006)
FBrf0218395-2896.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sbr 
unspecified role
sbr 
unspecified role
sbr 
unspecified role
Nup358 
unspecified role
Nup358 
unspecified role
Nup358 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 122.54377

FBrf0244040-19.PD.MW
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nup358 

Nup358

bait

GST tag

Nup358 
sbr 

dNxf1

prey
sbr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nup358 
FG domain
sufficient binding region
aa 2395-2426
sbr 
NTF2 and UBA domain
sufficient binding region
aa 359-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Nup358-sbr interaction may not be direct as Nxt1 was also added to the pull-down assay.

(Zhao et al., 2019)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhao et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)