FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
kst, α-Spec
FlyBase ID
FBig0000094054
Interaction Network
Interactions Browser links
kst network
α-Spec network
Reported Interactions
FBrf0131399-9.coIP.WB
Reference
Sisson et al., 2000
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 2embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic stage 4embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
α-Spec 

α-Spectrin

bait
kst 

βH-Spectrin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sisson et al., 2000)
FBrf0218395-6073.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kst 
unspecified role
kst 
unspecified role
kst 
unspecified role
α-Spec 
unspecified role
α-Spec 
unspecified role
α-Spec 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 344.26792

FBrf0227259-2.CH.MW
Reference
Khanna et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
α-Spec 

α

neutral component
α-Spec 

α

neutral component
α-Spec 
α-Spec 

α

neutral component
α-Spec 
kst 

βH

neutral component
kst 
kst 

βH

neutral component
kst 

βH

neutral component
kst 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
α-Spec 
N-terminal region
sufficient binding region
aa 2-152

"α 0-1" construct, coordinates relative to α-Spec-PA

kst 
C-terminal region
sufficient binding region
aa 3412-3594

"βH 31-32" construct, coordinates relative to kst-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Khanna et al., 2015)
FBrf0227259-4.ITC
Reference
Khanna et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kst 

βH

bait
kst 
α-Spec 

α

prey
α-Spec 
α-Spec 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
α-Spec 
N-terminal region
sufficient binding region
aa 2-152

"α 0-1" construct, coordinates relative to α-Spec-PA

kst 
C-terminal region
sufficient binding region
aa 3412-3594

"βH 31-32" construct, coordinates relative to kst-PA

α-Spec 
N-terminal region residue R22
mutation disrupting interaction
aa 22

R22S, coordinates relative to α-Spec-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Khanna et al., 2015)

Kd = 5 nM (at 23oC).

(Khanna et al., 2015)
FBrf0227970-2.coIP.WB
Reference
Fletcher et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
kst 

βH-Spec

bait

FLAG tag

kst 
α-Spec 

α-Spectrin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kst 
N-terminal region
sufficient binding region
aa 1-1632

coordinates relative to kst-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Fletcher et al., 2015)

Positive control.

(Fletcher et al., 2015)
FBrf0227970-5.coIP.WB
Reference
Fletcher et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kst 

βH-Spec

bait

GFP tag

α-Spec 

α-Spec

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Fletcher et al., 2015)

Positive control.

(Fletcher et al., 2015)
FBrf0232760-1.coIP.WB
Reference
Khanal et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kst 

βH-Spec

bait

YFP tag

α-Spec 

α-Spec

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Khanal et al., 2016)

Positive control.

(Khanal et al., 2016)
FBrf0239000-2.coIP.WB
Reference
Duan et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kst 

βH-spec

bait

FLAG tag

α-Spec 

α-Spec

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Duan et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)