PBL
fused to GAL4 DNA-binding domain
Rho1
fused to GAL4 activation domain
pbl protein interacts with Rho1, but not other Rho GTPases Rac1 or Cdc42.
Two-hybrid system: yeast GAL4-BD/GAL4-AD.
necessary: deletion abolishes interaction
Pbl
GST tag
GST tag, [3]H GDP label
[35]S label
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
pbl interacts more strongly with the GTP-bound form of Rho1 than the GDP-bound form.
