Cdc42, WASp Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

Cdc42, WASp

FlyBase ID

FBig0000094932

Interaction Network

Interactions Browser links

Cdc42 network
WASp network

Reported Interactions

FBrf0205746-9.ENZ

Reference

Rodal et al., 2008

Description

physical association

Assay

enzymatic study

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

WASp

Wsp

ancillary

His tag

WASp

Wsp

ancillary

His tag

WASp

Wsp

ancillary

His tag

Cdc42

ancillary

His tag

Cdc42

ancillary

His tag

Cdc42

ancillary

His tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

(Rodal et al., 2008)

Bovine Arp2/3 complex was mixed with pyrene labeled actin, to which WASp, nwk and Cdc42 were added in various combinations. Actin polymerization was monitored by fluorescence.

(Rodal et al., 2008)

FBrf0208460-2.PD.A

Reference

Liu et al., 2009

Description

physical association

Assay

pull down

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cdc42

bait

GST tag

WASp

Wasp

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Liu et al., 2009)

Positive control.

(Liu et al., 2009)

FBrf0209286-15.PD.WB

Reference

Fricke et al., 2009

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

Cell line used

S2R+

Participants

Corresponds to

Reported as

Role

Note

Cdc42

bait

GST tag

WASp

WASP

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2R+ cell extract.

(Fricke et al., 2009)

FBrf0211075-4.PD.WB

Reference

Nahm et al., 2010

Description

physical association

Assay

pull down

anti tag western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

WASp

Wsp

bait

GST tag

WASp

Cdc42

Cdc42V12

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

WASp

PRD

unspecified role

aa 301-378

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Bait expressed and purified from bacteria.

(Nahm et al., 2010)

Prey expressed in transiently transfected HEK293T cells.

(Nahm et al., 2010)

The interaction of WASp with the constitutively active Cdc42 V12 mutant is dependent upon the presence of wildtype Cip4 protein; the W603K Cip mutation, which disrupts the Cip SH3 domain, abolishes WASp-Cdc42 pull down.

(Nahm et al., 2010)

GST pull down.

(Nahm et al., 2010)

FBrf0227279-1.FRET.FM

Reference

Sharifai et al., 2014

Description

physical association

Assay

fluorescent resonance energy transfer

fluorescence microscopy

Collection

Source/Stage

larval central nervous system / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Cdc42

fluorescence donor

mEGFP tag

Cdc42

fluorescence donor

mEGFP tag

Cdc42

fluorescence donor

mEGFP tag

WASp

fluorescence acceptor

mCherry tag

WASp

fluorescence acceptor

mCherry tag

WASp

fluorescence acceptor

mCherry tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Sharifai et al., 2014)

FBrf0227279-2.FRET.FM

Reference

Sharifai et al., 2014

Description

physical association

Assay

fluorescent resonance energy transfer

fluorescence microscopy

Collection

Source/Stage

larval central nervous system / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

WASp

fluorescence donor

mEGFP tag

WASp

fluorescence donor

mEGFP tag

WASp

fluorescence donor

mEGFP tag

Cdc42

fluorescence acceptor

mCherry tag

Cdc42

fluorescence acceptor

mCherry tag

Cdc42

fluorescence acceptor

mCherry tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Sharifai et al., 2014)

External Crossreferences and Linkouts ( 1 )

Linkouts

MIST - An integrated Molecular Interaction Database

Cdc42,WASp

References (5)

Report Sections

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