Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type ovary extract.
Antibody supershift confirms that the BRE binding factor is aret protein.
Bait RNA derived from in vitro transcription.
Prey expressed from ovary cDNA in bacteria.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type ovary extract.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type ovarian extract.
Source was embryos of wild-type fly line; bait produced and labeled by in vitro transcription; prey produced from endogenous gene.
Positive control.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.
N521A,F523A, coordinates relative to bru1-PA
K239A,F241A, coordinates relative to bru1-PA
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.
S4E requires the mutation S7E to decrease the interaction.
S7E requires the mutation S4E to decrease the interaction.
T135E requires the mutations S4E and S7E to decrease the interaction.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type adult female ovary extract.
Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type adult female ovary extract.
Interaction in vitro; bait produced by in vitro transcription; prey produced by endogenous gene of ovary extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey synthesized and end-labeled in vitro.
Positive control.
Source was adult female ovary; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was adult female ovaries; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in Sf-21 cell system; prey produced and labeled by in vitro transcription.
CCGCGG to UUGCGG; within the context of nt 1-359
Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type ovary extract.
