FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
numb, Nak
FlyBase ID
FBig0000095038
Interaction Network
Interactions Browser links
numb network
Nak network
Reported Interactions
FBrf0099990-1.Y2H
Reference
Chien et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

fused to LexA DNA-binding domain

numb 
Nak 

Nak

prey

fused to GAL4 activation domain

Nak 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
unspecified role
aa 76-205
Nak 
C-terminal region
unspecified role
aa 1088-1490aa 1425-1490

necessary: deletion abolishes interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA/GAL4-AD.

(Chien et al., 1998)
FBrf0099990-2.PD.A
Reference
Chien et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nak 

Nak

bait

GST tag

Nak 
numb 

Numb

prey

[35]S label

numb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
unspecified role
aa 76-205
Nak 
Numb-interacting region
unspecified role
aa 1439-1449
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST pull down

(Chien et al., 1998)

Bait expressed and purified from bacteria.

(Chien et al., 1998)

Prey derived from in vitro translation.

(Chien et al., 1998)

Nak protein binds specifically to the PTB domain of numb but not of other PTB-containing proteins.

(Chien et al., 1998)
FBrf0099990-3.coIP.WB
Reference
Chien et al., 1998
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nak 

Nak

bait

myc tag

numb 

Numb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying Myc-tagged UAS-Nak construct, P{UAS-Nak.T:Myc}.

(Chien et al., 1998)

Prey endogenous to embryonic extracts.

(Chien et al., 1998)
FBrf0105874-1.FPS
Reference
Li et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

GST tag

numb 
Nak 

Nak-c

prey
Nak 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 58-205
Nak 
C-terminal peptide
sufficient binding region
GFSNMSFEDFP
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized.

(Li et al., 1998)
FBrf0184155-4.Y2H
Reference
Tang et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

fused to GAL4 DNA-binding domain

numb 
Nak 

Nak

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
phosphotyrosine binding (PTB) domain
mutation disrupting interaction
aa 195

F195V

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Tang et al., 2005)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Tang et al., 2005)
FBrf0222555-1.FPS
Reference
Zwahlen et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

GST tag

numb 
Nak 

Nak-c

prey
Nak 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 58-205
Nak 
C-terminal peptide
sufficient binding region
GFSNMSFEDFP
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized.

(Zwahlen et al., 2000)
FBrf0222555-2.NMR
Reference
Zwahlen et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait
numb 
Nak 

Nak-c

prey
Nak 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 58-205
Nak 
C-terminal peptide
sufficient binding region
GFSNMSFEDFP
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized.

(Zwahlen et al., 2000)
FBrf0238194-10.CS.MW
Reference
Shan et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

neutral component
numb 

Nb

neutral component
numb 
numb 

Nb

neutral component
numb 
Nak 

Nak

neutral component
Nak 
Nak 

Nak

neutral component
Nak 

Nak

neutral component
Nak 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 65-203

coordinates relative to numb-PA

Nak 
AB domain
sufficient binding region
aa 1431-1470

coordinates relative to Nak-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The estimated molecular weight of the numb PTB domain bound to Nak AB domain is consistent with a single heterodimer.

(Shan et al., 2018)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Shan et al., 2018)
FBrf0238194-8.PD.MW
Reference
Shan et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

bait

GST tag

numb 
Nak 

Nak

prey

His tag

Nak 
Nak 
Nak 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 65-203

coordinates relative to numb-PA

Nak 
AB domain
sufficient binding region
aa 1431-1470

coordinates relative to Nak-PA

Nak 
A domain
necessary binding region
aa 1431-1450

coordinates relative to Nak-PA

Nak 
B domain
necessary binding region
aa 1452-1470

coordinates relative to Nak-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Shan et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)