FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mon2, spir
FlyBase ID
FBig0000095079
Interaction Network
Interactions Browser links
mon2 network
spir network
Reported Interactions
FBrf0213769-1.Y2H
Reference
Tanaka et al., 2011
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mon2 

Mon2

bait
spir 

Spir

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Tanaka et al., 2011)

Prey library derived from ovarian cDNA.

(Tanaka et al., 2011)
FBrf0213769-2.Y2H
Reference
Tanaka et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mon2 

Mon2

bait
mon2 
spir 

Spir

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mon2 
region
unspecified role
aa 723-1160

\'Mon2-M\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Tanaka et al., 2011)

Prey was cloned reagent (previously isolated).

(Tanaka et al., 2011)
FBrf0213769-3.coIP.WB
Reference
Tanaka et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mon2 

Mon2

bait

GFP tag

spir 

Spir

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged mon2 and spir constructs.

(Tanaka et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)