Bait expressed in transiently transfected cells.

Prey expressed in transiently transfected cells.

Bait expressed and purified from bacteria.

Prey derived from in vitro translation.

Using [32]P labeled DNA with Trl binding sites and lacking ttk binding sites, the addition of ttk to Trl and DNA increased DNA-protein complexes observed by EMSA. This was taken as evidence of a Trl-ttk protein-protein interaction.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

HGScore = 14.956201

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).