FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Taf1, Tbp
FlyBase ID
FBig0000095629
Interaction Network
Interactions Browser links
Taf1 network
Tbp network
Reported Interactions
FBrf0065619-1.coIP.MW
Reference
Yokomori et al., 1993
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

TBP

bait
Taf1 

dTAFII250

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Yokomori et al., 1993)
FBrf0065619-25.coIP.WB
Reference
Yokomori et al., 1993
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf1 

dTAFII250

bait

HA tag

Tbp 

dTBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Taf1, Taf4, Taf12 and Tbp recombinant proteins were incubated and immunoprecipitated.

(Yokomori et al., 1993)

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus system; prey produced as a recombinant fusion protein in baculovirus system.

(Yokomori et al., 1993)
FBrf0074707-1.coIP.MW
Reference
Verrijzer et al., 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

TBP

bait
Taf1 

dTAF[[II]]250

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Verrijzer et al., 1994)
FBrf0078200-1.coIP.MW
Reference
Chen et al., 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

dTFIID

bait
Taf1 

dTAF[[II]]250

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Chen et al., 1994)
FBrf0089397-1a.PD.WB
Reference
Kokubo et al., 1994
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf1 

p230

bait

GST tag

Taf1 
Taf1 
Taf1 
Tbp 

TBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Taf1 
N-terminal portion
sufficient binding region
aa 2-81

Coordinates relative to Taf1-PA

Taf1 
N-terminal portion
mutation decreasing interaction
aa 19-23aa 67-71aa 24aa 26aa 29

clustered alanine substitutions. Coordinates relative to Taf1-PA

clustered alanine substitutions.Coordinates relative to Taf1-PA

L24A, Coordinates relative to Taf1-PA

G26A, Coordinates relative to Taf1-PA

I29A, Coordinates relative to Taf1-PA

Taf1 
N-terminal portion
mutation disrupting interaction
aa 24-28aa 26

clustered alanine substitutions. Coordinates relative to Taf1-PA

F26A, Coordinates relative to Taf1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced in baculovirus system.

(Kokubo et al., 1994)
FBrf0089397-2.PD.A
Reference
Kokubo et al., 1994
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

TBP

bait

Flag tag

Taf1 

p230

prey
Taf1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Taf1 
N terminally truncated protein
necessary binding region
aa 2-113

coordinates relative to Taf1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kokubo et al., 1994)
FBrf0137043-1.coIP.WB
Reference
Georgieva et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 11blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

TBP

bait
Taf1 

dTAF[[II]]230

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to embryonic nuclear extract.

(Georgieva et al., 2001)

Prey endogenous to embryonic nuclear extract.

(Georgieva et al., 2001)
FBrf0191293-7.CH.WB
Reference
Santoso and Kadonaga, 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf1 

TAF1

bait
Tbp 

TBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Santoso and Kadonaga, 2006)
FBrf0193855-1.coIP.WB
Reference
Katzenberger et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Taf1 

TAF1

bait

HA tag

Tbp 

TBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Katzenberger et al., 2006)

Taf1 isoform used, TAF1-4, corresponds to Taf1-PB or Taf1-PE which both contain exons 12a and 13a.

(Katzenberger et al., 2006)
FBrf0193855-2.coIP.WB
Reference
Katzenberger et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Taf1 

TAF1

bait
Tbp 

TBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Katzenberger et al., 2006)
FBrf0215303-7.CH.WB
Reference
Vorobyeva et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf1 

TAF1

neutral component
Taf1 

TAF1

neutral component
Taf1 

TAF1

neutral component
Tbp 

TBP

neutral component
Tbp 

TBP

neutral component
Tbp 

TBP

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; proteins produced from endogenous genes.

(Vorobyeva et al., 2009)
FBrf0227292-571.TFIN
Reference
Rhee et al., 2014
Description
physical association
Assay
Collection
TF_Interaction_Network-2
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Tbp 

FBgn0003687

unspecified role
Tbp 

FBgn0003687

unspecified role
Tbp 

FBgn0003687

unspecified role
Taf1 

FBgn0010355

unspecified role
Taf1 

FBgn0010355

unspecified role
Taf1 

FBgn0010355

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

(Rhee et al., 2014)

HGScore = 15.88399

(Rhee et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (9)