FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Rel, dl
FlyBase ID
FBig0000095642
Interaction Network
Interactions Browser links
Rel network
dl network
Reported Interactions
FBrf0218496-5.SPR
Reference
Bitra et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rel 

Rel-49

bait

His tag

Rel 
dl 

Dorsal

prey

Strep tag

dl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dl 
Rel homology domain
sufficient binding region
aa 47-360
Rel 
ankyrin repeat domain
sufficient binding region
aa 546-971

His tag

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

dl homodimers tested in this assay.

(Bitra et al., 2012)

Bait expressed and purified from bacteria.

(Bitra et al., 2012)

Prey expressed and purified from bacteria.

(Bitra et al., 2012)

The Rel ankyrin repeat domain (Rel-49) binds the Rel homology domain of dl more strongly than that of Rel or Dif.

(Bitra et al., 2012)
FBrf0241691-2.coIP.WB
Reference
Chowdhury et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rel 

Rel

bait

V5 or FLAG tag

Rel 
dl 

Dl

prey

V5 or FLAG tag

dl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rel 
RHD domain
sufficient binding region
aa 152-335

coordinates relative to Rel-PA

dl 
RHD domain
sufficient binding region
aa 49-219

coordinates relative to dl-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chowdhury et al., 2019)
FBrf0241691-4.coIP.WB
Reference
Chowdhury et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
dl 

Dl

bait

V5 or FLAG tag

dl 
Rel 

Rel

prey

V5 or FLAG tag

Rel 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rel 
RHD domain
sufficient binding region
aa 152-335

coordinates relative to Rel-PA

dl 
RHD domain
sufficient binding region
aa 49-219

coordinates relative to dl-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chowdhury et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)