FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Patj, sdt
FlyBase ID
FBig0000095643
Interaction Network
Interactions Browser links
Patj network
sdt network
Reported Interactions
FBrf0204906-1.Y2H
Reference
Bulgakova et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

fused to GAL4 DNA-binding domain

sdt 
Patj 

Patj

prey

fused to GAL4 activation domain

Patj 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
L27 domain
sufficient binding region
aa 566-681

coordinates relative to sdt-PB

Patj 
N-terminal region
sufficient binding region
aa 1-87

coordinates relative to Patj-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bulgakova et al., 2008)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bulgakova et al., 2008)
FBrf0212253-1.coIP.WB
Reference
Bulgakova et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

FLAG tag

Patj 

PATJ

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Bulgakova et al., 2010)

One isoform of tagged sdt does not coimmunoprecipitate other endogenous sdt isoforms.

(Bulgakova et al., 2010)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bulgakova et al., 2010)
FBrf0217316-11.coIP.WB
Reference
Pocha et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Patj 

DPatj

bait
sdt 

Sdt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head lysate.

(Pocha et al., 2011)
FBrf0217316-15.coIP.WB
Reference
Pocha et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait
Patj 

DPatj

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head lysate.

(Pocha et al., 2011)
FBrf0217316-3.coIP.WB
Reference
Pocha et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Patj 

DPatj

bait
sdt 

Sdt

prey

41 peptides recovered

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head lysate.

(Pocha et al., 2011)
FBrf0217316-7.coIP.WB
Reference
Pocha et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait
Patj 

DPatj

prey

57 peptides recovered

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head lysate.

(Pocha et al., 2011)
FBrf0228512-1.coIP.WB
Reference
Sen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Patj 

Patj

bait

GFP tag

Patj 
sdt 

Sdt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Patj 
L27 domain
necessary binding region
aa

"ΔL27" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sen et al., 2015)
FBrf0234434-4.coIP.WB
Reference
Koch et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait
Patj 

PATJ

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Koch et al., 2016)
FBrf0234434-8.coIP.WB
Reference
Koch et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sdt 

Sdt

bait

GFP tag

sdt 
Patj 

PATJ

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
L27N domain
necessary binding region
aa 193-250

"ΔL27N" construct, coordinates relative to sdt-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Koch et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)