FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
His3, brm
FlyBase ID
FBig0000095664
Interaction Network
Interactions Browser links
His3 network
brm network
Reported Interactions
FBrf0218406-16.PD.WB
Reference
Tie et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait

GST tag

brm 

BRM

prey

His tag

brm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brm 
bromodomain-containing C-terminus
sufficient binding region
aa 1417-1578
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST pull down.

(Tie et al., 2012)

Bait expressed and purified from bacteria.

(Tie et al., 2012)

Prey expressed and purified from bacteria.

(Tie et al., 2012)

brm does not bind the His3 N-terminal tail in this assay.

(Tie et al., 2012)
FBrf0248694-1.PD.WB
Reference
Regadas et al., 2021
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait

biotin tag

His3 
brm 

Brm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His3 
N-terminal domain
sufficient binding region
aa 3-19
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Regadas et al., 2021)
FBrf0248694-3.PD.MS
Reference
Regadas et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait

biotin tag

His3 
brm 

Brm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His3 
N-terminal domain
sufficient binding region
aa 3-19
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell nuclear extract.

(Regadas et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)