Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/VP16-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Mutant Smn shows reduced binding to wild type Smn as compared to binding of wild type Smn to wild type Smn.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

This experiment used wild type Smn as bait and both wild type and mutant Smn as prey.

Bait expressed in transfected cells.

Prey expressed in transfected cells.

D20V and F70S mutations do not affect Smn self-oligomerization.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Smn found to be oligomeric.

Interaction in vitro; proteins produced as recombinant fusion proteins in a bacterial system.