Kd = 0.12uM

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized.

Bait expressed and purified in bacteria.

Prey derived from in vitro translation.

Positive control.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Caspase-cleaved grim retains its ability to bind th.

Increased expression of th increases grim ubiquitination in an eff-dependent (UbcD1) manner

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

grim can interact with both the BIR1 and BIR2 domains of th.

When the BIR2 domain of th is mutated, and grim can only bind to the BIR1 domain, th apparently auto-ubiquitinates itself.