smo, Usp8 Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

smo

bait

Myc tag

smo

Usp8

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

smo

C-terminal domain

sufficient binding region

aa 556-1035

smo

C-terminal domain

necessary binding region

aa 556-1035

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2012)

FBrf0218247-1.coIP.WB

Reference

Xia et al., 2012

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

smo

Smo

bait

Myc tag

smo

Usp8

USP8

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

smo

C-terminal region

sufficient binding region

aa 556-730aa 731-1035

weak interaction

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Bait expressed in transfected cells.

(Xia et al., 2012)

Prey expressed in transfected cells.

(Xia et al., 2012)

FBrf0218247-2.coIP.WB

Reference

Xia et al., 2012

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

smo

Smo

bait

Myc tag

smo

Usp8

USP8

prey

HA tag

Usp8

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

smo

C-terminal region

sufficient binding region

aa 556-1035

smo

PKA phosphorylation sites

mutation decreasing interaction

aa 667-740

S667A, S687A and S740A mutations abolish interaction

smo

PKA and CK1 phosphorylation sites

mutation increasing interaction

aa 667-743

mutation of Serine residues to Aspartate increases interaction

Usp8

C-terminal region

sufficient binding region

aa 536-896

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Bait expressed in transfected cells.

(Xia et al., 2012)

Prey expressed in transfected cells.

(Xia et al., 2012)

Mutations that mimic the phosphorylated state the smo C-terminal region enhance the smo-Ubpy interaction.

(Xia et al., 2012)

FBrf0218247-3.PD.WB

Reference

Xia et al., 2012

Description

physical association

Assay

pull down

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

smo

Smo

bait

GST tag

smo

Usp8

USP8

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

smo

C-terminal region

sufficient binding region

aa 625-753

smo

PKA and CK1 phosphorylation sites

mutation increasing interaction

aa 667-743

mutation of Serine residues to Aspartate increases interaction

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

GST pull down.

(Xia et al., 2012)

Bait expressed and purified from bacteria.

(Xia et al., 2012)

Prey expressed in transfected cells.

(Xia et al., 2012)

Treatment of S2 cells with hh-containing conditioned medium increases the interaction of Ubpy with GST-smo.

(Xia et al., 2012)

Mutations that mimic the phosphorylated state the smo C-terminal region enhance the smo-Ubpy interaction.

(Xia et al., 2012)

FBrf0223342-3.coIP.WB

Reference

Fan et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

smo

Smo

bait

Myc tag

Usp8

USP8

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fan et al., 2013)

The binding of Ubpy to smo is not affected by knockdown or over-expression of Hrs.

(Fan et al., 2013)

FBrf0235873-2.coIP.WB

Reference

Ma et al., 2016

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

smo

Smo

bait

Myc tag

Usp8

prey

FLAG tag

Usp8

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Usp8

interaction domain

mutation disrupting interaction

aa 260,262,263,498,500,501,780,782,783

I260K,I262K,V263K,V498K,V500K,V501K,V780K,V782K,V783K

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ma et al., 2016)

FBrf0246976-12.coIP.WB

Reference

Li et al., 2020

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

smo

Smo

bait

Myc tag

Usp8

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2020)

Co-expression of Rack1 increases the smo-Usp8 interaction.

(Li et al., 2020)

FBrf0246976-9.coIP.WB

Reference

Li et al., 2020

Description

physical association

Assay

Collection

Source/Stage

Cell line used