FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
l(2)gd1, shrb
FlyBase ID
FBig0000095714
Interaction Network
Interactions Browser links
l(2)gd1 network
shrb network
Reported Interactions
FBrf0217584-1.coIP.WB
Reference
Troost et al., 2012
Description
physical association
Assay
Collection
Source/Stage
/ third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gd1 

Lgd

bait

HA tag

shrb 

Shrub

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a HA-tagged l(2)gd1 construct, P{UAS-lgd.HA.J}.

(Troost et al., 2012)

Prey derived from a transgenic line carrying a GFP-tagged shrb construct, P{UAS-shrb-GFP}.

(Troost et al., 2012)
FBrf0217584-2.coIP.WB
Reference
Troost et al., 2012
Description
physical association
Assay
Collection
Source/Stage
/ third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shrb 

Shrub

bait

GFP tag

l(2)gd1 

Lgd

prey

HA tag

l(2)gd1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
l(2)gd1 
DM14 domains
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a GFP-tagged shrb construct, P{UAS-shrb-GFP}.

(Troost et al., 2012)

Prey derived from a transgenic line carrying various HA-tagged l(2)gd1 constructs.

(Troost et al., 2012)
FBrf0217584-3.coIP.WB
Reference
Troost et al., 2012
Description
physical association
Assay
Collection
Source/Stage
/ third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gd1 

Lgd

bait

HA tag

shrb 

Shrub

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a HA-tagged l(2)gd1 construct, P{UAS-lgd.HA.J}.

(Troost et al., 2012)

Prey endogenous to larval extract.

(Troost et al., 2012)
FBrf0217584-4.PD
Reference
Troost et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shrb 

Shrub

bait

GST tag

l(2)gd1 

Lgd

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Troost et al., 2012)

Prey expressed and purified from bacteria.

(Troost et al., 2012)

GST pull down.

(Troost et al., 2012)
FBrf0238233-1.SPR
Reference
McMillan et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shrb 

Shrub

bait
shrb 
shrb 
l(2)gd1 

Lgd

prey
l(2)gd1 
l(2)gd1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shrb 
interaction domain
sufficient binding region
aa 6-106
shrb 
interaction domain residue 86
mutation disrupting interaction
aa 86

E86R

l(2)gd1 
DM14-3 domain
sufficient binding region
aa 359-423
l(2)gd1 
DM14-3 domain residue 393
mutation disrupting interaction
aa 393

R393A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(McMillan et al., 2017)

Kd = 2.96 uM

(McMillan et al., 2017)
FBrf0238233-2.XC
Reference
McMillan et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shrb 

Shrub

neutral component
shrb 

Shrub

neutral component
shrb 
shrb 

Shrub

neutral component
shrb 
l(2)gd1 

Lgd

neutral component
l(2)gd1 
l(2)gd1 

Lgd

neutral component
l(2)gd1 

Lgd

neutral component
l(2)gd1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shrb 
interaction domain
sufficient binding region
aa 17-130
l(2)gd1 
DM14-3 domain
sufficient binding region
aa 359-422
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(McMillan et al., 2017)

Due to difficulty crystallizing a stable shrb-l(2)gd1 complex, shrb and l(2)gd1 were produced as a single fusion protein with mutations R59E and R70E in shrb to suppress polymerization and an 11 residue glycine linker.

(McMillan et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)