FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nej, brm
FlyBase ID
FBig0000095722
Interaction Network
Interactions Browser links
nej network
brm network
Reported Interactions
FBrf0218406-1.coIP.WB
Reference
Tie et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

CBP

bait
brm 

BRM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to embryonic nuclear extract.

(Tie et al., 2012)

Prey endogenous to embryonic nuclear extract.

(Tie et al., 2012)
FBrf0218406-11.PD.WB
Reference
Tie et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

CBP

bait

GST tag

nej 
nej 
brm 

BRM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
zinc finger 1
sufficient binding region
aa 302-758
nej 
PHD domain
sufficient binding region
aa 1805-1993
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST pull down.

(Tie et al., 2012)

Bait expressed and purified from bacteria.

(Tie et al., 2012)

Prey endogenous to embryonic nuclear extract.

(Tie et al., 2012)

The zinc finger 1 and PHD domains of nej can interact independently with brm.

(Tie et al., 2012)
FBrf0218406-15.PD.WB
Reference
Tie et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

CBP

bait

GST tag

brm 

brm

prey

His tag

brm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brm 
bromodomain-containing C-terminus
sufficient binding region
aa 1417-1578
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST pull down.

(Tie et al., 2012)

Bait expressed and purified from bacteria.

(Tie et al., 2012)

Prey expressed and purified from bacteria.

(Tie et al., 2012)
FBrf0218406-5.coIP.WB
Reference
Tie et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

BRM

bait
nej 

CBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to embryonic nuclear extract.

(Tie et al., 2012)

Prey endogenous to embryonic nuclear extract.

(Tie et al., 2012)
FBrf0218406-8.CH.WB
Reference
Tie et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

CBP

neutral component
nej 

CBP

neutral component
nej 

CBP

neutral component
brm 

BRM

neutral component
brm 

BRM

neutral component
brm 

BRM

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

nej, brm and Utx cofractionate on gel filtration columns in a complex with apparent molecular weight of 2MDa.

(Tie et al., 2012)

Bait endogenous to embryonic nuclear extract.

(Tie et al., 2012)

Prey endogenous to embryonic nuclear extract.

(Tie et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)